Neonatal calf diarrhea is a frequent disease of calves and may result in dehydration and metabolic acidosis. The disease causes mortality and reduces growth and future productivity. Early identification of disease improves calf outcomes and thus there is increasing interest in technological methods for detecting disease. Dehydration leads to the blood becoming more concentrated and this can be measured using serum osmolality. Research in humans has shown that saliva conductivity is correlated with serum osmolality. Saliva conductivity may therefore offer a non-invasive opportunity to assess hydration status in calves. Furthermore, as blood pH is a prognostic indicator and there is ion exchange in the salivary ducts, saliva pH may act as an indicator of metabolic acidosis. This observational study aimed to assess the relationship of saliva conductivity and pH with the clinical and biochemical parameters of calves suffering from neonatal calf diarrhea. One hundred and forty-one dairy-bred calves were recruited onto the study at approximately 1 week of age. The health of the calves was assessed daily. Calves had blood and saliva samples taken weekly until 25 days of age or the development of neonatal calf diarrhea. When calves developed diarrhea, they were sampled for three consecutive days. Hematocrit, plasma total protein, saliva pH and saliva conductivity were measured at each sampling. Saliva pH and saliva conductivity were measured using portable meters ( and ). In a subset of 30 matched samples, serum proteins and electrolytes were also measured. Saliva conductivity was not associated with diarrhea or dehydration. Saliva pH was lower in calves with diarrhea, regardless of hydration status. The Lin's concordance correlation coefficients between saliva variables and hematocrit and strong ion difference were negligible. Dehydrated calves with diarrhea had a higher hematocrit and albumin and the lowest sodium and SID. Calves with diarrhea and no dehydration had a lower plasma total protein. While saliva conductivity has been associated with measures of dehydration in humans, this does not appear to be the case in calves. Saliva pH has not previously been considered for disease detection; however as it is associated with diarrhea, further research is warranted.