Brus-Szkalej Maja, Dotson Bradley, Andersen Christian B, Vetukuri Ramesh R, Grenville-Briggs Laura J
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 190, 234 22 Lomma, Sweden.
Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 190, 234 22 Lomma, Sweden.
Phytopathology. 2025 Apr;115(4):374-386. doi: 10.1094/PHYTO-03-24-0107-R. Epub 2025 Apr 25.
Transglutaminases (TGases) are enzymes highly conserved among prokaryotic and eukaryotic organisms, where their role is to catalyze protein cross-linking. One of the putative TGases of has previously been shown to be localized to the cell wall. Based on sequence similarity, we were able to identify six more genes annotated as putative TGases and show that these seven genes group together in phylogenetic analysis. These seven proteins are predicted to contain both a TGase domain and a MANSC domain, the latter of which was previously shown to play a role in protein stability. Chemical inhibition of TGase activity and silencing of the entire family of the putative cell wall TGases are both lethal to . , indicating the importance of these proteins in cell wall formation and stability. The intermediate phenotype obtained with lower drug concentrations and less efficient silencing displays a number of deformations to germ tubes and appressoria. Both chemically treated and silenced lines show lower pathogenicity than the wild type in leaf infection assays. Finally, we show that appressoria of . possess the ability to build up turgor pressure and that this ability is decreased by chemical inhibition of TGases. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
转谷氨酰胺酶（TGases）是在原核生物和真核生物中高度保守的酶，其作用是催化蛋白质交联。之前已证明其中一种假定的TGases定位于细胞壁。基于序列相似性，我们能够鉴定出另外六个注释为假定TGases的基因，并表明这七个基因在系统发育分析中聚集在一起。预计这七种蛋白质都含有一个TGase结构域和一个MANSC结构域，后者先前已证明在蛋白质稳定性中起作用。TGase活性的化学抑制和假定的细胞壁TGases整个家族的沉默对……都是致命的，这表明这些蛋白质在细胞壁形成和稳定性中的重要性。用较低药物浓度和较低效率沉默获得的中间表型显示出生殖管和附着胞有许多变形。在叶片感染试验中，经化学处理和沉默的品系均显示出比野生型更低的致病性。最后，我们表明……的附着胞具有建立膨压的能力，并且这种能力会因TGases的化学抑制而降低。[公式：见正文] 版权所有© 2025作者。这是一篇根据知识共享署名4.0国际许可协议分发的开放获取文章。
