通过FLP-FRT和CRISPR/Cas9重排果蝇中Hox复合体的顺式调控模块

Re-arranging the Cis-regulatory Modules of Hox Complex in Drosophila via FLP-FRT and CRISPR/Cas9.

作者信息

Hajirnis Nikhil

机构信息

Department of Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD, USA.

出版信息

Methods Mol Biol. 2025;2889:11-24. doi: 10.1007/978-1-0716-4322-8_2.

DOI:10.1007/978-1-0716-4322-8_2

PMID:39745602

Abstract

FLP-FRT, a well-established technique for genome manipulation, and the revolutionary CRISPR/Cas9, known for its targeted indels, are combined in a novel approach. This unique method is applied to the Hox genes in the Drosophila melanogaster bithorax complex, which are closely located to the cis-regulatory modules that define their spatial-temporal regulation. The number and position of these genes are directly correlated to their expression pattern. This chapter unveils the exciting potential of this combinatorial use of FLP-FRT and CRISPR-Cas9 to rearrange the cis-regulatory modules of the Hox complex in Drosophila melanogaster.

摘要

FLP-FRT是一种成熟的基因组操作技术，而以靶向插入缺失闻名的革命性CRISPR/Cas9技术，则以一种新颖的方法与之相结合。这种独特的方法应用于黑腹果蝇双胸复合体中的Hox基因，这些基因与定义其时空调控的顺式调控模块紧密相邻。这些基因的数量和位置与其表达模式直接相关。本章揭示了FLP-FRT和CRISPR-Cas9组合使用在重排黑腹果蝇Hox复合体顺式调控模块方面令人兴奋的潜力。