Newman C N, Miller J H
Mutat Res. 1985 Jan-Mar;145(1-2):95-101. doi: 10.1016/0167-8817(85)90046-x.
Several laboratories have reported that exposure of cells to UV radiation results in a significant imbalance in deoxynucleoside triphosphate pool concentrations. In our CHO-K1 cells, a rapid drop in dCTP is accompanied by a rapid increase in dTTP. Examination of enzyme activities associated with synthesis/degradation of these molecules suggests that UV transiently enhances a putative dCTPase, dCMP deaminase and CdR kinase activities. This results in accumulation of excess dUMP which is probably converted to dTMP, then to dTTP. The absence of dCMP deaminase in V79 cells prohibits this rapid response in those cells. Moreover, significantly different dCMP deaminase activities were observed in CHO-K1 cells obtained from other laboratories, suggesting they, too, may respond differently to irradiation.
几个实验室报告称，将细胞暴露于紫外线辐射会导致脱氧核苷三磷酸池浓度出现显著失衡。在我们的中国仓鼠卵巢细胞（CHO-K1）中，脱氧胞苷三磷酸（dCTP）迅速下降，同时脱氧胸苷三磷酸（dTTP）迅速增加。对与这些分子合成/降解相关的酶活性进行检测表明，紫外线会短暂增强一种假定的dCTP酶、脱氧胞苷一磷酸脱氨酶和胞嘧啶阿拉伯糖苷激酶的活性。这会导致过量脱氧尿苷一磷酸（dUMP）积累，而dUMP可能会转化为脱氧胸苷一磷酸（dTMP），进而转化为dTTP。V79细胞中缺乏脱氧胞苷一磷酸脱氨酶，这使得这些细胞无法产生这种快速反应。此外，在从其他实验室获得的CHO-K1细胞中观察到了显著不同的脱氧胞苷一磷酸脱氨酶活性，这表明它们对辐射的反应可能也有所不同。
