Lin Fei, Zheng Wen-Cai, Ke Zhi-Bin, Chen Dong-Ning, Xue Yu-Ting, Lin Yun-Zhi, Li Xiao-Dong, Zheng Qing-Shui, Wei Yong, Xue Xue-Yi, Xu Ning
Department of Urology, Urology Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Urology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, China.
Department of Urology, Urology Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Urology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, China; Fujian Key Laboratory of Precision Medicine for Cancer, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China.
Environ Pollut. 2025 Feb 15;367:125625. doi: 10.1016/j.envpol.2024.125625. Epub 2024 Dec 31.
Di-(2-ethylhexyl) phthalate (DEHP) is widely utilized as a plasticizer in industrial manufacturing to enhance the durability and flexibility of plastics. Studies have depicted that DEHP exposure may be associated with multiple cancers, including colorectal, liver and prostate cancer. However, the effects and underlying mechanisms of DEHP on bladder cancer progression remain unspecific. In this work, we explored the correlation between DEHP exposure and bladder cancer through comprehensive analysis. A total of 172 differentially expressed DEHP-related genes (DEDRGs) were screened based on the CTD and TCGA database. In addition, a new prognostic model related to DEHP was developed, which had excellent predictive power for bladder cancer prognoses. Molecular docking techniques were employed to assess the binding affinity of mono(2-ethylhexyl) phthalate (MEHP) to crucial proteins. Lastly, both in vitro and in vivo experiments, along with RNA sequencing, were conducted to elucidate the biological roles and mechanisms of MEHP in bladder cancer. Several new insights into the role of DEHP/MEHP in bladder cancer were given in this study, as well as an awareness of the association between environmental toxicants and cancer progression.
邻苯二甲酸二（2-乙基己基）酯（DEHP）在工业生产中被广泛用作增塑剂，以提高塑料的耐用性和柔韧性。研究表明，接触DEHP可能与多种癌症有关，包括结直肠癌、肝癌和前列腺癌。然而，DEHP对膀胱癌进展的影响及其潜在机制仍不明确。在这项工作中，我们通过综合分析探讨了DEHP暴露与膀胱癌之间的相关性。基于CTD和TCGA数据库筛选出总共172个差异表达的DEHP相关基因（DEDRGs）。此外，还开发了一种与DEHP相关的新预后模型，该模型对膀胱癌预后具有出色的预测能力。采用分子对接技术评估单（2-乙基己基）邻苯二甲酸酯（MEHP）与关键蛋白的结合亲和力。最后，进行了体外和体内实验以及RNA测序，以阐明MEHP在膀胱癌中的生物学作用和机制。本研究对DEHP/MEHP在膀胱癌中的作用有了一些新的认识，同时也提高了对环境毒物与癌症进展之间关联的认识。
