Kumrungsee Nutchaya, Nobsathian Saksit, Chumworathayee Worakawee, Phankaen Poonnanan, Dunkhunthod Benjawan, Koul Opender, Saiyaitong Chatwadee, Bullangpoti Vasakorn
Biology Department, Faculty of Science and Technology, Rajamangala University of Technology, Thanyaburi, Pathumthani, 12110, Thailand.
Animal Toxicology and Physiology Speciality Research Unit, Zoology Department, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Sci Rep. 2025 Jan 2;15(1):27. doi: 10.1038/s41598-024-83351-1.
The brown planthopper (BPH) Nilaparvata lugens (Stål) is a major insect pest of Oryza sativa that causes crop yield loss in tropical regions, including Thailand. In this study, the crude ethanolic extract of the leaves and branches of Combretum trifoliatum , its active isolated components, apigenin and camphor, and Finopril were tested for their ability to control the first to fifth instars of N. lugens. The C. trifoliatum crude extract and both allelochemicals showed insecticide potential (24 h-LC50 ~ 8.83-95.96 mg/L against each instar for crude extract), and their toxicity depended on the time of exposure. Camphor showed the higher efficacy (LD50 ~ 4.43 mg/L) and not different compared to Finopril. All plant compounds tested reduced carboxylesterase (CE) and glutathione-s-transferase (GST) activities. Camphor caused the greatest decreases in CE and GST activities after exposure, whereas apigenin induced a slight change in acetylcholinesterase activity. The results of the present study suggest that C. trifoliatum extract can be used as an insecticide to manage N. lugens populations.
褐飞虱（Nilaparvata lugens (Stål)）是水稻的一种主要害虫，在包括泰国在内的热带地区会导致作物减产。在本研究中，对三叶风车子叶和枝条的乙醇粗提物、其活性分离成分芹菜素和樟脑以及杀螟硫磷控制褐飞虱一至五龄若虫的能力进行了测试。三叶风车子粗提物和两种化感物质均显示出杀虫潜力（粗提物对各龄期的24小时半数致死浓度约为8.83 - 95.96毫克/升），且其毒性取决于暴露时间。樟脑显示出更高的功效（半数致死剂量约为4.43毫克/升），与杀螟硫磷相比无差异。所有测试的植物化合物均降低了羧酸酯酶（CE）和谷胱甘肽 - S - 转移酶（GST）的活性。暴露后，樟脑导致CE和GST活性下降幅度最大，而芹菜素使乙酰胆碱酯酶活性发生轻微变化。本研究结果表明，三叶风车子提取物可作为杀虫剂用于防治褐飞虱种群。
