Ghebosu Raluca E, Hui Lawrence, Wolfram Joy
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, 4072, Australia.
School of Chemical Engineering, The University of Queensland, Brisbane, QLD, 4072, Australia.
J Biomed Sci. 2025 Jan 3;32(1):3. doi: 10.1186/s12929-024-01095-6.
The biomolecular relevance of medium supplements is a key challenge affecting cell culture practice. The biomolecular composition of commonly used supplements differs from that of a physiological environment, affecting the validity of conclusions drawn from in vitro studies. This article discusses the advantages and disadvantages of common supplements, including context-dependent considerations for supplement selection to improve biomolecular relevance, especially in nanomedicine and extracellular vesicle research.
培养基补充剂的生物分子相关性是影响细胞培养实践的关键挑战。常用补充剂的生物分子组成与生理环境不同，影响了体外研究得出结论的有效性。本文讨论了常见补充剂的优缺点，包括在选择补充剂时根据具体情况进行考量以提高生物分子相关性，特别是在纳米医学和细胞外囊泡研究中。
