Li Huaixin, Wu Mingli, Chao Hongbo, Yin Yongtai, Xia Yutian, Cheng Xin, Chen Kang, Yan Shuxiang, Wang Xiaodong, Xiong Yiyi, He Jianjie, Fan Shipeng, Ding Yiran, Zhang Libin, Jia Haibo, Zhang Chunyu, Li Maoteng
College of Life Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Sci Adv. 2025 Jan 3;11(1):eads7620. doi: 10.1126/sciadv.ads7620.
Yellow seed coat color (SCC) is a valuable trait in , which is significantly correlated to high seed oil content (SOC) and low seed lignocellulose content (SLC). However, no dominant yellow SCC genes were identified in . In this study, a dominant yellow SCC N53-2 was verified, and then 58,981 eQTLs and 25 trans-eQTL hotspots were identified in a double haploid population derived from N53-2 and black SCC material Ken-C8. A rare dominant allele () was subsequently cloned in a trans-eQTL hotspot that colocated with SCC, SOC, and SLC QTL hotspot on ChrA09 through QTL fine mapping and multi-omics analysis. Transgenic experiments revealed that the expression of produced yellow SCC seeds with significantly increased SOC and decreased SLC. Our result provides a rare dominant yellow SCC allele in , which has excellent potential for yellow SCC and high SOC rapeseed breeding.
黄色种皮颜色（SCC）是油菜中的一个重要性状，与高种子油含量（SOC）和低种子木质纤维素含量（SLC）显著相关。然而，在油菜中尚未鉴定出显性黄色SCC基因。本研究中，验证了一个显性黄色SCC品系N53-2，随后在由N53-2和黑色SCC材料Ken-C8构建的双单倍体群体中鉴定出58,981个eQTL和25个反式eQTL热点区域。通过QTL精细定位和多组学分析，在A09染色体上一个与SCC、SOC和SLC QTL热点区域共定位的反式eQTL热点区域中克隆到一个罕见的显性等位基因（）。转基因实验表明，该基因的表达产生了黄色SCC种子，其SOC显著增加，SLC显著降低。我们的研究结果为油菜提供了一个罕见的显性黄色SCC等位基因，在黄色SCC和高SOC油菜育种方面具有良好的应用潜力。
