Martinez Aldair, Lavergne Emma, Brauge Clément, Laveran Emma, Bertagnoli Stéphane, Boucraut-Baralon Corine, Bessière Pierre
IHAP, Université de Toulouse, INRAE, ENVT, Toulouse, France.
Scanelis - Cerba Vet Laboratory, Colomiers, France.
Vet Microbiol. 2025 Feb;301:110355. doi: 10.1016/j.vetmic.2024.110355. Epub 2024 Dec 28.
Feline coronavirus (FCoV) is a virus endemic in cat populations. Specific genomic mutations give it a strong tropism for macrophages, allowing systemic infection and the development of a disease known as feline infectious peritonitis. This disease takes various clinical presentations, and can manifest as uveitis. Two mutations in the spike protein have been identified as possibly associated with FIP: mutations M1058L and S1060A. 193 clinical samples of aqueous humor were collected, all PCR-positive for feline coronavirus. Samples were taken either from cats with a clinical picture compatible with an ocular form of FIP (with uveitis and general clinical signs), or from cats with uveitis only. We sequenced the region of the S gene coding for positions 1058 and 1060 for 77 samples. The aim of the study was to determine whether viruses from cats with clinical signs compatible with FIP were more likely to harbor the M1058L and S1060A mutations. Our results confirm that these mutations are associated with severe disease, and also show that ocular samples from cats with uveitis alone are more likely to contain FECV.
猫冠状病毒（FCoV）是猫群体中的一种地方性病毒。特定的基因组突变使其对巨噬细胞具有强烈的嗜性，从而导致全身感染并引发一种名为猫传染性腹膜炎的疾病。这种疾病有多种临床表现，可表现为葡萄膜炎。刺突蛋白中的两种突变已被确定可能与猫传染性腹膜炎有关：M1058L和S1060A突变。收集了193份房水临床样本，所有样本经聚合酶链反应检测猫冠状病毒均呈阳性。样本取自具有与眼部形式的猫传染性腹膜炎相符的临床症状（伴有葡萄膜炎和一般临床体征）的猫，或仅患有葡萄膜炎的猫。我们对77个样本中编码第1058和1060位的S基因区域进行了测序。该研究的目的是确定来自具有与猫传染性腹膜炎相符的临床症状的猫的病毒是否更有可能携带M1058L和S1060A突变。我们的结果证实这些突变与严重疾病有关，并且还表明仅患有葡萄膜炎的猫的眼部样本更有可能含有猫肠道冠状病毒。
