Xiang Zhipan, Zhang Lin, Zhang Mingze, Yao Yuxian, Qian Qianqian, Wei Ziyi, Cui Baolu, Wang Dengyan, Quan Changbin, Lu Minfeng, Chen Liangbi
School of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun, 558000, China; Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Plant Physiol Biochem. 2025 Mar;220:109455. doi: 10.1016/j.plaphy.2024.109455. Epub 2024 Dec 25.
Cold stress is one of the most serious abiotic stresses that affects the growth and yield in rice. However, the molecular mechanism by which abscisic acid (ABA) regulates plant cold stress tolerance is not yet clear. In this study, we identified a member of the OsNCED (9-cis-epoxycarotenoid dioxygenase) gene family, OsNCED5, which confers cold stress tolerance in rice. OsNCED5 encodes a chloroplast-localized ABA biosynthetic enzyme and its expression is strongly induced by cold stress. Disruption of OsNCED5 by CRISPR/Cas9-mediated mutagenesis led to a significant decrease in ABA content and exhibited significant reduced cold stress tolerance at the seedling stage. Exogenous ABA restored the cold stress tolerance of the osnced5 mutants. Overexpression of OsNCED5 gene significantly improved the cold stress tolerance of rice seedlings. Moreover, OsNCED5 mainly regulates cold stress tolerance through regulating reactive oxygen species (ROS) homeostasis. Taken together, we identified a new OsNCED regulator involved in cold stress tolerance, and provided a potential target gene for enhancing cold stress tolerance in rice.
冷胁迫是影响水稻生长和产量的最严重非生物胁迫之一。然而，脱落酸（ABA）调节植物耐冷性的分子机制尚不清楚。在本研究中，我们鉴定出水稻OsNCED（9-顺式环氧类胡萝卜素双加氧酶）基因家族的一个成员OsNCED5，它赋予水稻耐冷性。OsNCED5编码一种定位于叶绿体的ABA生物合成酶，其表达受冷胁迫强烈诱导。通过CRISPR/Cas9介导的诱变破坏OsNCED5导致ABA含量显著降低，并在苗期表现出明显降低的耐冷性。外源ABA恢复了osnced5突变体的耐冷性。OsNCED5基因的过表达显著提高了水稻幼苗的耐冷性。此外，OsNCED5主要通过调节活性氧（ROS）稳态来调节耐冷性。综上所述，我们鉴定出一个参与耐冷性的新OsNCED调节因子，并为提高水稻耐冷性提供了一个潜在的靶基因。
