Wang Wenlin, Zhou Mo, Xu Shaohui, Huang Zhong, Li Xiaobei, Zhou Cai, Wang Siming, Zhang Kaiyuan, Qiao Lixian, Tang Yanyan
College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China.
Plants (Basel). 2025 Jun 6;14(12):1741. doi: 10.3390/plants14121741.
Peanut ( L.), a vital oilseed and cash crop, faces yield limitations due to abiotic stresses. The 9--epoxycarotenoid dioxygenase (NCED) enzyme, a key enzyme in abscisic acid (ABA) biosynthesis regulating plant development and stress responses, remains mechanistically uncharacterized in peanut abiotic stress tolerance. In this study, we isolated a novel gene, , from the salt-tolerant mutant M24. The expression of was strongly induced by NaCl, PEG6000, and ABA in peanut huayu20. Overexpression of enhanced salt and drought tolerance in . Transgenic overexpression of improved salt and stress resistance through upregulated ROS-scavenging genes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) with elevated enzymatic activities while reducing malondialdehyde (MDA), superoxide anion (O), and hydrogen peroxide (HO) accumulation compared to wild-type plants. Further research showed that the chlorophyll fluorescence parameters of transgenic lines were significantly increased, while light damage was significantly reduced. These findings establish as a critical regulator of stress adaptation and an excellent candidate gene for resistance breeding in peanut.
花生（Arachis hypogaea L.）是一种重要的油料和经济作物，由于非生物胁迫而面临产量限制。9-顺式环氧类胡萝卜素双加氧酶（NCED）是脱落酸（ABA）生物合成中的关键酶，调控植物发育和胁迫反应，但其在花生非生物胁迫耐受性中的作用机制仍不清楚。在本研究中，我们从耐盐突变体M24中分离出一个新基因AhNCED1。AhNCED1的表达在花生花育20中受到NaCl、PEG6000和ABA的强烈诱导。AhNCED1的过表达增强了拟南芥的耐盐性和耐旱性。与野生型植株相比，AhNCED1的转基因过表达通过上调活性氧清除基因超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的表达，提高了酶活性，同时减少了丙二醛（MDA）、超氧阴离子（O₂⁻）和过氧化氢（H₂O₂）的积累，从而提高了耐盐性和抗逆性。进一步研究表明，转基因株系的叶绿素荧光参数显著增加，而光损伤显著降低。这些发现确立了AhNCED1作为胁迫适应的关键调节因子以及花生抗性育种的优良候选基因的地位。
