Fang Yikun, Zhang Yan, Liu Caiyun, Zhu Hanchuang, Wang Kun, Ma Lixue, Li Xinke, Rong Xiaodi, Li Wenzhai, Sheng Wenlong, Zhu Baocun
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 5;330:125634. doi: 10.1016/j.saa.2024.125634. Epub 2024 Dec 21.
As a heavy metal contaminant, mercury ion (Hg) has caused great harm to environment and life. Mercury ions will migrate and transform in the environment and eventually accumulate in the human body, thus causing human poisoning. Therefore, it is of great significance to detect Hg in the environment and living bodies. Based on this, we constructed an example of reactive near-infrared ratiometric fluorescent probe NIRPC-Hg based on the ICT-ESIPT mechanism. The probe could not only effectively overcome the effects of environmental changes, thus realizing the accurate detection of Hg in the environment, but also possessed the advantages of large emission wavelength shift (130 nm red shift). In addition, the probe NIRPC-Hg was constructed with the classical recognition structure of Hg and NIR fluorophore, which could detect Hg sensitively with a low detection limit (9.21 nM). The satisfactory experimental results of environmental water samples proved that the probe had high application potential in the environment. At the same time, the probe NIRPC-Hg had the characteristics of smaller photo-toxicity and lower autofluorescence interference in the bioimaging applications, which could conveniently realize the sensitive detection of Hg in cells and zebrafish. Therefore, the construction of probe NIRPC-Hg provided an effective tool to detect Hg in the environment and living organisms.
作为一种重金属污染物，汞离子（Hg）对环境和生命造成了极大危害。汞离子会在环境中迁移和转化，最终在人体中积累，从而导致人体中毒。因此，检测环境和生物体内的汞具有重要意义。基于此，我们构建了一种基于ICT-ESIPT机制的反应型近红外比率荧光探针NIRPC-Hg。该探针不仅能有效克服环境变化的影响，从而实现对环境中汞的准确检测，还具有发射波长红移大（130 nm红移）的优点。此外，探针NIRPC-Hg采用了汞的经典识别结构与近红外荧光团构建而成，能以低检测限（9.21 nM）灵敏地检测汞。环境水样的实验结果令人满意，证明该探针在环境中具有很高的应用潜力。同时，探针NIRPC-Hg在生物成像应用中具有光毒性较小和自发荧光干扰较低的特点，能够方便地实现对细胞和斑马鱼中汞的灵敏检测。因此，探针NIRPC-Hg的构建为检测环境和生物体内的汞提供了一种有效工具。
