Roger Sarah, Estevez Samantha, Hernandez-Nieto Carlos, Kripalani Shawn, Ghofranian Atoosa, Baird Morgan, Lee Joseph, Slifkin Richard, Briton-Jones Christine, Copperman Alan, Danis Rachel
Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
Reproductive Medicine Associates of New York, New York, NY, USA.
J Assist Reprod Genet. 2025 Feb;42(2):525-532. doi: 10.1007/s10815-024-03350-z. Epub 2025 Jan 3.
This study is to evaluate duration of oocyte cryostorage and association with thaw survival, fertilization, blastulation, ploidy rates, and pregnancy outcomes in patients seeking fertility preservation.
Retrospective cohort study to evaluate clinical outcomes in patients who underwent fertility preservation from 2011 to 2023 via oocyte vitrification for non-oncologic indications. Primary outcome was thaw survival rate. Secondary outcomes included rates of fertilization, blastulation and embryo ploidy, as well asn pregnancy outcomes. Demographic, controlled ovarian stimulation, and laboratory data were collected for each patient. Analyses were conducted using Kruskal-Wallis, Fisher's exact test, or Chi-squared test, as appropriate. A multivariate logistic regression analysis was utilized, and adjusted odds ratios with 95% confidence intervals were calculated using Group A as the reference group.
A total of 5995 oocytes met inclusion criteria. These were divided into quartiles based on duration of cryostorage. Group A included oocytes in cryostorage for 0-2 years; Group B, 3-5 years; and Group C, 6-11 years. Significant differences were found when comparing thaw survival (p < 0.0001), fertilization (p = 0.0009), and blastulation rates (p = 0.0002) among groups; however, no significant differences were seen after multivariate GEE regression analysis. No significant differences were seen in rates of euploidy (p = 0.70), live birth (p = 0.81), and clinical pregnancy (p = 0.58) among groups. After analyzing years in cryostorage as a continuous variable and controlling for confounders, duration of cryostorage was not related to odds of thaw, fertilization, blastulation, euploid rates, or pregnancy outcomes.
Length of time in cryostorage is not associated with compromised rates of oocyte thaw survival, fertilization, blastulation, ploidy, or pregnancy outcomes.
本研究旨在评估卵母细胞冷冻保存的时长及其与解冻存活率、受精率、囊胚形成率、倍性率以及寻求生育力保存患者的妊娠结局之间的关联。
进行回顾性队列研究，以评估2011年至2023年期间因非肿瘤适应症通过卵母细胞玻璃化进行生育力保存的患者的临床结局。主要结局为解冻存活率。次要结局包括受精率、囊胚形成率和胚胎倍性率，以及妊娠结局。收集了每位患者的人口统计学、控制性卵巢刺激和实验室数据。根据情况使用Kruskal-Wallis检验、Fisher精确检验或卡方检验进行分析。采用多因素逻辑回归分析，并以A组作为参照组计算调整后的比值比及95%置信区间。
共有5995个卵母细胞符合纳入标准。根据冷冻保存时长将其分为四分位数。A组包括冷冻保存0至2年的卵母细胞；B组为3至5年；C组为6至11年。比较各组间的解冻存活率（p < 0.0001）、受精率（p = 0.0009）和囊胚形成率（p = 0.0002）时发现存在显著差异；然而，多因素广义估计方程回归分析后未发现显著差异。各组间的整倍体率（p = 0.70）、活产率（p = 0.81）和临床妊娠率（p = 0.58）未发现显著差异。将冷冻保存年限作为连续变量进行分析并控制混杂因素后，冷冻保存时长与解冻、受精、囊胚形成、整倍体率或妊娠结局的几率无关。
冷冻保存时长与卵母细胞解冻存活率、受精率、囊胚形成率、倍性率或妊娠结局降低无关。
