Wang Jinlei, Li Ke, Yuan Haizhong
PKUCare Lu'an Hospital, 046204, Shanxi, China.
2nd Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China.
Sci Rep. 2025 Jan 4;15(1):800. doi: 10.1038/s41598-025-85123-x.
Periodontitis, a common chronic inflammatory condition caused by bacteria, leads to loss of attachment, resorption of alveolar bone, and ultimately tooth loss. Therefore, reducing bacterial load and fostering alveolar bone regeneration are essential components in the treatment of periodontitis. In this study, we prepared smaller-sized Ag-Metal Organic Frameworks (Ag@MOF) and loaded with sodium alginate (Alg) hydrogel for periodontitis treatment. The results showed that Ag@MOF with a smaller particle size was prepared, approximately 5.5 nm. It successfully hindered the development of Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) by disrupting bacterial intracellular metabolism, generating ROS, compromising cell membrane integrity, and preventing biofilm formation. The Ag@MOF/Alg hydrogel displayed a characteristic interconnected three-dimensional structure, along with hydrophilic and antimicrobial effects. The Ag@MOF/Alg hydrogel we developed greatly enhances the invasion and migration capabilities of endothelial cells, as well as promoting angiogenesis. In mouse models of periodontitis induced by ligature, the extent of bone loss in the jaw and the presence of cells causing inflammation in the tissues surrounding the teeth were improved in the group treated with Ag@MOF/Alg hydrogel. The levels of TNF-α, IL-6, and IL-1β were significantly reduced compared to the control group. Conclusion: The experimental results prove that Ag@MOF/Alg hydrogel offers a new therapeutic approach for periodontitis.
牙周炎是一种由细菌引起的常见慢性炎症性疾病，会导致附着丧失、牙槽骨吸收，最终导致牙齿脱落。因此，降低细菌载量和促进牙槽骨再生是牙周炎治疗的重要组成部分。在本研究中，我们制备了尺寸更小的银-金属有机框架（Ag@MOF）并负载海藻酸钠（Alg）水凝胶用于牙周炎治疗。结果表明，制备出了粒径约为5.5纳米的较小粒径的Ag@MOF。它通过破坏细菌细胞内代谢、产生活性氧、损害细胞膜完整性和防止生物膜形成，成功阻碍了大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）的生长。Ag@MOF/Alg水凝胶呈现出典型的相互连接的三维结构，同时具有亲水性和抗菌作用。我们开发的Ag@MOF/Alg水凝胶极大地增强了内皮细胞的侵袭和迁移能力，以及促进血管生成。在结扎诱导的牙周炎小鼠模型中，用Ag@MOF/Alg水凝胶治疗的组中颌骨骨丢失程度以及牙齿周围组织中引起炎症的细胞数量均有所改善。与对照组相比，肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的水平显著降低。结论：实验结果证明Ag@MOF/Alg水凝胶为牙周炎提供了一种新的治疗方法。
