Sun Xiaohan, Zhang Nanxin, Chen Longhui, Lai Yuchao, Yang Shasha, Li Qiang, Zheng Yunquan, Chen Li, Shi Xianai, Yang Jianmin
College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China.
Department of Orthopedics, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Fuzhou 350004, China.
Biomater Adv. 2025 Apr;169:214164. doi: 10.1016/j.bioadv.2024.214164. Epub 2024 Dec 29.
In anterior cruciate ligament (ACL) repair methods, the continuous enzymatic erosion of synovial fluid can impede healing and potentially lead to repair failure, as well as exacerbate articular cartilage wear, resulting in joint degeneration. Inspired by the blood clot during medial collateral ligament healing, we developed a composite scaffold comprising collagen (1 %, w/v) and polyvinyl alcohol (5 %, w/v) combined with platelet-rich plasma (PRP). The composite scaffold provides a protective barrier against synovial erosion for the ruptured ACL, while simultaneously facilitating tissue repair, thereby enhancing the efficacy of ACL repair techniques. The composite scaffold is primarily formed through hydrogen bonding between molecular chains and physical cross-linking of microcrystalline regions using a simple cyclic freeze-thaw method, resulting in improved mechanical properties and an extended degradation period. The maximum tensile fracture load of the composite scaffold reached 5.99 ± 0.30 N. The incorporation of PRP facilitates cell migration, proliferation, and blood vessel growth by enabling slow release of various growth factors. In vivo results demonstrate that this composite scaffold promotes rabbit hindlimb rupture ACL healing by stimulating fibroblast proliferation, collagen deposition, microvascular formation, and proprioceptor generation. Furthermore, it effectively reduces meniscus and cartilage wear while mitigating bone arthritis and joint degenerative diseases. Overall, our proposed composite scaffold holds great promise as a candidate for ACL healing.
在前交叉韧带（ACL）修复方法中，滑液的持续酶促侵蚀会阻碍愈合，并可能导致修复失败，还会加剧关节软骨磨损，导致关节退变。受内侧副韧带愈合过程中血凝块的启发，我们开发了一种由胶原蛋白（1%，w/v）和聚乙烯醇（5%，w/v）与富血小板血浆（PRP）组合而成的复合支架。该复合支架为破裂的ACL提供了一个防止滑膜侵蚀的保护屏障，同时促进组织修复，从而提高ACL修复技术的疗效。该复合支架主要通过分子链之间的氢键和采用简单的循环冻融方法使微晶区域进行物理交联而形成，从而改善了机械性能并延长了降解期。复合支架的最大拉伸断裂载荷达到5.99±0.30 N。PRP的加入通过使各种生长因子缓慢释放，促进细胞迁移、增殖和血管生长。体内结果表明，这种复合支架通过刺激成纤维细胞增殖、胶原蛋白沉积、微血管形成和本体感受器生成，促进兔后肢破裂ACL的愈合。此外，它有效地减少了半月板和软骨磨损，同时减轻了骨关节炎和关节退行性疾病。总体而言，我们提出的复合支架作为ACL愈合的候选材料具有很大的潜力。
