Liu Xiaoyan, Li Aowei, Yang Xueying, Luo Guanghong, Zhu Jianbo
Gansu Engineering Technology Research Center for Microalgae, Hexi University, Zhangye, 734000, China; College of Life Sciences, Shihezi University, Shihezi, 832000, China.
College of Life Sciences, Shihezi University, Shihezi, 832000, China.
Plant Physiol Biochem. 2025 Mar;220:109440. doi: 10.1016/j.plaphy.2024.109440. Epub 2024 Dec 26.
Pyrus sinkiangensis, a crucial economic fruit tree in Xinjiang, China, experiences winter hardiness that significantly influences its yield and fruit quality. This study aimed to investigate the role of PsHB7/12 in cold resistance of Pyrus sinkiangensis and its regulation of abscisic acid (ABA) signaling. Through physiological assessments and transcriptome analysis, we identified a peak expression of PsHB7/12 in January, which was strongly induced by ABA. We found a correlation between ABA concentrations and changes in water content and soluble protein levels during overwintering process. Further analysis of yeast one-hybrid and the luciferase assay revealed that PsHB7/12 was involved in the negative regulation of ABA signal by inhibiting the expression of PsPYL4. Additionally, the overexpression of the PsHB7/12 may have complex effects on ABA signaling through the modulation of expression of members of the PsPP2Cs family. In summary, PsHB7/12 regulates the ABA signaling pathway through a negative feedback mechanism. These findings reveal the critical role of PsHB7/12 in cold stress adaptation in Pyrus sinkiangensis and provide new molecular markers for breeding stress-resistant fruit trees.
新疆梨是中国新疆一种重要的经济果树，其抗寒性对产量和果实品质有显著影响。本研究旨在探究PsHB7/12在新疆梨抗寒中的作用及其对脱落酸（ABA）信号的调控。通过生理评估和转录组分析，我们发现PsHB7/12在1月表达量达到峰值，且受ABA强烈诱导。我们发现越冬过程中ABA浓度与水分含量及可溶性蛋白水平的变化之间存在相关性。进一步的酵母单杂交和荧光素酶检测分析表明，PsHB7/12通过抑制PsPYL4的表达参与ABA信号的负调控。此外，PsHB7/12的过表达可能通过调节PsPP2Cs家族成员的表达对ABA信号产生复杂影响。总之，PsHB7/12通过负反馈机制调控ABA信号通路。这些发现揭示了PsHB7/12在新疆梨适应冷胁迫中的关键作用，并为培育抗逆果树提供了新的分子标记。
