Ning Qing, Yue Yingxue, Liu Xinlian, Ji Wenbo, Zhao Qin, Wang Jing, Zhang Zhenhai
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
State Key Laboratory of Oral Drug Delivery Systems of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, China.
Phytother Res. 2025 Mar;39(3):1344-1354. doi: 10.1002/ptr.8426. Epub 2025 Jan 6.
Safe and effective treatments for psoriasis are limited. Anisole is an active ingredient in citrus and basil volatile oils; however, its potential for psoriasis treatment remains unexplored. To investigate the effects and mechanism of anisole transdermal administration as a treatment for psoriasis. Imiquimod (IMQ) was used to establish a C57 mouse psoriasis model. The severity of psoriasis lesions in each group was assessed by evaluating the thickness of skin lesions, erythema, and scales. Pathological changes within the epidermis organization were evaluated via hematoxylin and eosin (H&E) staining using light microscopy. Serum inflammatory factor levels were measured by enzyme-linked immunosorbent assays. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB) were performed to analyze the mRNA levels of relevant inflammatory factors and the expression of key proteins at the skin lesion sites in psoriatic mice. In model mice, applying anisole hydrogels significantly reduced the serum levels of pro-inflammatory factors interleukin (IL)-17A, (IL)-23, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ. H&E staining revealed significantly attenuated stratum spinosum thickening and lymphocyte infiltration in the treatment group. RT-qPCR results further demonstrated that the skin tissues from the treatment group exhibited significantly reduced Il1b, Il17a, Il22, and Tnfa mRNA expression. Western blotting revealed inhibition of the JAK1/STAT3 signaling pathway within anisole-treated psoriatic tissues. Anisole potentially reduces psoriatic-associated inflammation through the JAK1/STAT3 signaling pathway, alleviating IMQ-induced psoriasis.
治疗银屑病的安全有效方法有限。茴香脑是柑橘和罗勒挥发油中的一种活性成分；然而，其治疗银屑病的潜力尚未得到探索。为了研究茴香脑经皮给药治疗银屑病的效果和机制。使用咪喹莫特（IMQ）建立C57小鼠银屑病模型。通过评估皮肤病变的厚度、红斑和鳞屑来评估每组银屑病病变的严重程度。使用苏木精和伊红（H&E）染色，通过光学显微镜评估表皮组织内的病理变化。通过酶联免疫吸附测定法测量血清炎症因子水平。进行实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹法（WB），以分析银屑病小鼠皮肤病变部位相关炎症因子的mRNA水平和关键蛋白的表达。在模型小鼠中，应用茴香脑水凝胶可显著降低促炎因子白细胞介素（IL）-17A、（IL）-23、肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ的血清水平。H&E染色显示治疗组棘层增厚和淋巴细胞浸润明显减轻。RT-qPCR结果进一步表明，治疗组皮肤组织中Il1b、Il17a、Il22和Tnfa mRNA表达显著降低。蛋白质印迹法显示茴香脑处理的银屑病组织中JAK1/STAT3信号通路受到抑制。茴香脑可能通过JAK1/STAT3信号通路减轻银屑病相关炎症，缓解IMQ诱导的银屑病。
