Wu Jingtao, Xia Yonghui, Wang Tianyu, Zhang Yafeng, Li Guangli
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.
Zhuzhou Institute for Food and Drug Control, Zhuzhou 412000, China.
Food Chem X. 2024 Dec 14;25:102094. doi: 10.1016/j.fochx.2024.102094. eCollection 2025 Jan.
An ultrasensitive and selective voltammetric platform combined a molecularly imprinted poly(pyrrole) membrane with Ag-nanoparticle-functionalized black phosphorus nanosheets (MIP/BPNS-AgNPs) was developed for trace GAT detection. The physicochemical properties of the MIP/BPNS-AgNPs were studied by various spectroscopic and electrochemical techniques. BPNS-AgNPs improved the ambient stability and electrochemical activity of the BPNS and possessed a large surface area for accommodating abundant templates to produce specific imprinted sites. The resulting MIP/BPNS-AgNP-modified glassy carbon electrode (GCE) greatly enhanced voltammetric responses for GAT. The MIP/BPNS-AgNP/GCE exhibited admirable GAT determination performance, with two linear responses (0.001-1 and 1-50 μM), high sensitivity (9.965 and 0.5378 μA μM), and a low detection limit of 0.2 nM. In addition, the MIP electrode could selectively detect GAT in complex matrices and retain roust responses for a month. The applicability of MIP/BPNS-AgNP/GCE toward the detection of GAT in pharmaceutical formulations, milk, and human serum was verified with satisfactory results.
开发了一种超灵敏且选择性的伏安检测平台，该平台将分子印迹聚吡咯膜与银纳米颗粒功能化的黑磷纳米片（MIP/BPNS-AgNPs）相结合用于痕量γ-氨基丁酸（GAT）检测。通过各种光谱和电化学技术研究了MIP/BPNS-AgNPs的物理化学性质。BPNS-AgNPs提高了BPNS的环境稳定性和电化学活性，并具有大的表面积以容纳大量模板从而产生特定的印迹位点。所得的MIP/BPNS-AgNP修饰玻碳电极（GCE）极大地增强了对GAT的伏安响应。MIP/BPNS-AgNP/GCE表现出令人满意的GAT测定性能，具有两个线性响应范围（0.001 - 1和1 - 50 μM）、高灵敏度（9.965和0.5378 μA μM⁻¹）以及0.2 nM的低检测限。此外，该MIP电极能够在复杂基质中选择性检测GAT，并在一个月内保持稳定响应。验证了MIP/BPNS-AgNP/GCE在药物制剂、牛奶和人血清中检测GAT的适用性，结果令人满意。
