Okamoto S, Handa H, Handa Y
Surg Neurol. 1985 Apr;23(4):421-4. doi: 10.1016/0090-3019(85)90222-8.
The release of prostaglandinlike substances from canine pial arteries that was induced by exposure of the pial arteries to red blood cell hemolysate was estimated by using a superfusion technique for prostaglandin bioassay. The assay organs used were strips of rat stomach for prostaglandin E2- or prostaglandin F2 alpha-like substances, strips of dog coronary artery for prostaglandin I2-like substance, and strips of dog ileum for prostaglandin F2 alpha-like substance. The substances released from the canine pial arteries induced contractions in rat stomach strips and relaxations in canine coronary arterial strips, whereas they did not induce any response in canine ileal strips. The equivalent prostaglandin E2 doses for the contractions of the rat stomach strips and the equivalent prostaglandin I2 doses for the relaxations of the canine coronary arterial strips were 125.2 +/- 19.4 (n = 8) and 59.5 +/- 16.4 (n = 6) pmol/g wet wt +/- SEM, respectively.
采用前列腺素生物测定的灌流技术，对犬软脑膜动脉暴露于红细胞溶血产物时所诱导的类前列腺素物质释放进行了评估。所使用的测定器官包括用于检测前列腺素E2或类前列腺素F2α物质的大鼠胃条、用于检测类前列腺素I2物质的犬冠状动脉条以及用于检测类前列腺素F2α物质的犬回肠条。犬软脑膜动脉释放的物质可引起大鼠胃条收缩以及犬冠状动脉条舒张，而对犬回肠条未引起任何反应。引起大鼠胃条收缩的等效前列腺素E2剂量以及引起犬冠状动脉条舒张的等效前列腺素I2剂量分别为125.2±19.4（n = 8）和59.5±16.4（n = 6）pmol/g湿重±标准误。
