Hu Jun, Ba Yina, Pan Zhifeng, Li Xiaogang
Shanghai Electric Power Hospital, Shanghai, China.
School of Life Science, Fudan University, Shanghai, China.
Heliyon. 2024 Nov 26;10(24):e40629. doi: 10.1016/j.heliyon.2024.e40629. eCollection 2024 Dec 30.
Exposure to low doses of antibiotics in organisms may have long-term effects on host growth and brain neurochemicals, which are achieved by disrupting the composition and metabolism of gut flora. Therefore, we should pay more attention to the use and management of antibiotics to protect human health and the ecological environment. Here, we developed a method of detecting 50 antibiotic residues simultaneously in human plasma using HPLC-MS/MS. We optimized the sample pre-treatment method, chromatographic and MS parameters. The best elution buffer was 60 % acetonitrile, which ensured high recovery rate of antibiotics. The main seven kinds of antibiotics, including β-lactams, tetracyclines, macrolides, lincosamides, chloramphenicol, sulfonamides, quinolones could be detected by this method. The average recovery rate was 67.25 %-129.03 %. Analytes have been detected with limit of detection (LOD) values from 0.1 ng ml to 5 ng ml. In brief, the method is reliable and robust for rapid screening of antibiotic residues, which was suitable and efficient to monitor antimicrobial exposure.
生物体接触低剂量抗生素可能会对宿主生长和大脑神经化学物质产生长期影响，这是通过破坏肠道菌群的组成和代谢来实现的。因此，我们应该更加关注抗生素的使用和管理，以保护人类健康和生态环境。在此，我们开发了一种利用高效液相色谱-串联质谱法（HPLC-MS/MS）同时检测人血浆中50种抗生素残留的方法。我们优化了样品预处理方法、色谱和质谱参数。最佳洗脱缓冲液为60%乙腈，这确保了抗生素的高回收率。该方法可检测包括β-内酰胺类、四环素类、大环内酯类、林可酰胺类、氯霉素类、磺胺类、喹诺酮类在内的七种主要抗生素。平均回收率为67.25%-129.03%。检测到的分析物的检测限（LOD）值为每毫升0.1纳克至5纳克。简而言之，该方法对于快速筛查抗生素残留是可靠且稳健的，适用于高效监测抗菌药物暴露情况。
