Fan Zhiyi, Zhang Guofu, Zhan Wanda, Wang Juehan, Wang Chaoyong, Yue QianYing, Huang Zhangheng, Wang Yongxiang
Northern Jiangsu People's Hospital, Yangzhou, Jiangsu, China.
Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China.
Mater Today Bio. 2024 Dec 6;30:101378. doi: 10.1016/j.mtbio.2024.101378. eCollection 2025 Feb.
Spinal cord injury (SCI) is a neurological condition that causes significant loss of sensory, motor, and autonomic functions below the level of injury. Current clinical treatment strategies often fail to meet expectations. Hyaluronidase is typically associated with tumor progression and bacterial infections. Analysis showed that hyaluronidase also persistently increased in a rat total excision model. In this study, we designed a highly biocompatible dual-responsive hydrogel. Hyaluronic acid (HA)-Gelatin (Gel) served as the base for the hydrogel, crosslinked via an amide reaction to form the hydrogel. The hydrogel was further combined with Neurotrophic growth factor (NGF) and FeO nanoparticles, exhibiting low toxicity, good mechanical properties, self-healing ability, and sustained drug release. In cellular experiments, the novel hydrogel significantly promoted neural axon growth and development under an external magnetic field. Therapeutic results were confirmed in a rat spinal cord resection model, where inflammation was reduced, chondroitin sulfate proteoglycans decreased and a favorable environment for nerve regeneration was provided; neural regeneration improved hind limb motor function in SCI rats. These results underscore the therapeutic potential of hydrogel.
脊髓损伤（SCI）是一种神经疾病，会导致损伤平面以下的感觉、运动和自主神经功能显著丧失。目前的临床治疗策略往往未能达到预期效果。透明质酸酶通常与肿瘤进展和细菌感染有关。分析表明，在大鼠全切模型中透明质酸酶也持续增加。在本研究中，我们设计了一种具有高度生物相容性的双响应水凝胶。透明质酸（HA）-明胶（Gel）作为水凝胶的基质，通过酰胺反应交联形成水凝胶。该水凝胶进一步与神经营养生长因子（NGF）和FeO纳米颗粒结合，表现出低毒性、良好的机械性能、自愈能力和药物缓释性能。在细胞实验中，新型水凝胶在外部磁场作用下显著促进神经轴突的生长和发育。在大鼠脊髓切除模型中证实了治疗效果，炎症减轻，硫酸软骨素蛋白聚糖减少，为神经再生提供了有利环境；神经再生改善了SCI大鼠的后肢运动功能。这些结果强调了水凝胶的治疗潜力。
