Shirzadi-Ahodashti Mina, Ebrahimzadeh Mohammad Ali, Biparva Pourya
Ramsar Campus, Mazandaran University of Medical Sciences, Ramsar, Iran.
Pharmaceutical Sciences Research Center, and Department of Medicinal Chemistry, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
J Fluoresc. 2025 Jan 6. doi: 10.1007/s10895-024-04115-8.
Among the various cations, the Fe ion is one of the most critical transition metal ions in living cells for many cellular functions and enzymatic activities. The decrease or overloading of Fe can lead to different disruptions in humans. Also, Fe, highly toxic, is very common in all industrial wastewater. So, the design and synthesis of Fe chelators as high-selective chemosensors are essential for detecting Fe. In this paper, 6-(6-bromopyridin-2-yl)benzo[4,5]imidazo[1,2-c]quinazoline (BQ) as a novel colorimetric sensor for sensing of Fe was synthesized and characterized by H-NMR, C-NMR, IR, and mass spectrometry. The sensing behavior of BQ toward various cations has been analyzed via colorimetric detection and UV-vis spectroscopy. The BQ was observed to have excellent selectivity and sensitivity to the presence of Fe among the tested ions in aqueous media. Interestingly, the BQ can selectively recognize Fe ions with the naked eye via the color change of colorless to yellow. The effect of pH on the stability and absorption of BQ towards Fe was studied. The results of pH studies showed that the absorbance of complex BQ/Fe remains stable between pH 5-10. Moreover, to determine the sensing behavior of BQ to Fe, the mole ratio method was carried out. The binding stoichiometry of BQ/Fe was assessed by using Job's plot and indicated the 1:1 binding ratio between BQ and Fe. Under the optimal conditions, there was a good linear relationship (correlation coefficient R = 0.9886) in Fe concentration in the 58-114 μM range, with a detection limit of 53.39 nM.
在各种阳离子中，铁离子是活细胞中许多细胞功能和酶活性的最关键过渡金属离子之一。铁含量的减少或过载会导致人体出现不同的紊乱。此外，具有高毒性的铁在所有工业废水中都很常见。因此，设计和合成作为高选择性化学传感器的铁螯合剂对于检测铁至关重要。本文合成了6-(6-溴吡啶-2-基)苯并[4,5]咪唑并[1,2-c]喹唑啉（BQ）作为一种新型的用于检测铁的比色传感器，并通过氢核磁共振、碳核磁共振、红外光谱和质谱对其进行了表征。通过比色检测和紫外可见光谱分析了BQ对各种阳离子的传感行为。观察到BQ在水性介质中对测试离子中的铁具有优异的选择性和灵敏度。有趣的是，BQ可以通过从无色到黄色的颜色变化用肉眼选择性地识别铁离子。研究了pH对BQ对铁的稳定性和吸收的影响。pH研究结果表明，配合物BQ/Fe的吸光度在pH 5至pH 10之间保持稳定。此外，为了确定BQ对铁的传感行为，采用了摩尔比法。通过Job曲线评估BQ/Fe的结合化学计量，表明BQ与铁的结合比为1:1。在最佳条件下，铁浓度在58 - 114 μM范围内存在良好的线性关系（相关系数R = 0.9886），检测限为53.39 nM。
