Pharmaceutical Sciences Research Center, School of Pharmacy, Hemoglobinopathy Institute and Department of Medicinal Chemistry, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, P.O.Box 578, Sari, Iran.
J Fluoresc. 2022 Nov;32(6):2309-2318. doi: 10.1007/s10895-022-03016-y. Epub 2022 Sep 17.
A novel quinazoline-based colorimetric sensor (probe 1) that detected Fe via naked-eye in a buffered MeOH: HO (8:2) solvent system within a neutral pH range was synthesized and its structure was confirmed by H-NMR, C-NMR, FT-IR, and Mass spectroscopy. Its photophysical properties were also studied. The sensitivity and selectivity factor in the presence of 16 metal ions was examined by utilizing absorption titrations. Based on the selectivity test, probe 1 exhibited excellent selectivity and sensitivity toward Fe ions among various other ions. The competitive effect indicated relatively low interference of other cations on the interaction of probe 1/Fe. To specify the sensing behavior of probe 1 to Fe , the mole ratio method was carried out. After addition of around 500 µL of Fe, the absorption of probe1 reached saturation, and the reaction was completed. The effect of pH on the absorption and stability of probe 1 towards Fe was examined. The pH range from 5.0 to 9.0 was appropriate for detection of Fe. To find the binding stoichiometric of the complex, Job's plot studies were carried out by varying the molar ratio of Fe. A 1:2 binding ratio was proposed. Under the optimal conditions, a good linear relationship (R = 0.9886) was at the concentration of Fe over the range of 50-110 µM, with a detection limit of 47.44 nM. Our quinazoline-based probe has shown good results for the quantitative determination of Fe in samples of urine with high recovery.
一种新型基于喹唑啉的比色传感器（探针 1），在中性 pH 值范围内，在缓冲的 MeOH:HO（8:2）溶剂体系中通过裸眼检测 Fe，其结构通过 H-NMR、C-NMR、FT-IR 和质谱法确认。还研究了其光物理性质。通过吸收滴定法检查了在存在 16 种金属离子时的灵敏度和选择性因子。基于选择性测试，探针 1 在各种其他离子中对 Fe 离子表现出优异的选择性和灵敏度。竞争效应表明，其他阳离子对探针 1/Fe 相互作用的干扰相对较低。为了指定探针 1 对 Fe 的感测行为，进行了摩尔比法。加入约 500 µL 的 Fe 后，探针 1 的吸收达到饱和，反应完成。还检查了 pH 值对探针 1 对 Fe 的吸收和稳定性的影响。pH 值范围为 5.0 至 9.0 适用于 Fe 的检测。为了找到配合物的结合化学计量比，通过改变 Fe 的摩尔比进行了 Job 图研究。提出了 1:2 的结合比。在最佳条件下，在 50-110 µM 的范围内，Fe 的浓度与良好的线性关系（R = 0.9886），检测限为 47.44 nM。我们基于喹唑啉的探针在尿液样品中对 Fe 的定量测定中表现出良好的效果，回收率高。
