Xin Yin, Pan Wenqiang, Zhao Yajie, Yang Chenglong, Li Jingru, Wang Shaokun, Wu Jingxiang, Zhang Mingfang, Shi Jinxin, Ma Yang, Fang Shaozhong, Liang Yuwei, Zaccai Michele, Zhang Xiuhai, Du Yunpeng, Wu Jian
Institute of Grassland, Flowers, and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China.
Plant Physiol. 2025 Jan 6. doi: 10.1093/plphys/kiaf001.
Trichomes play a crucial role in plant resistance to abiotic and biotic stresses, and their development and characteristics vary across different species. This study demonstrates that trichomes of Lilium pumilum exhibit synchronized growth during flower bud differentiation and enhance the plant's adaptability to UV-B radiation and aphid infection. We identified LpNAC48, a NAC family transcription factor (TF), that interacted with the B-box (BBX) family TF LpBBX28, during trichome formation in L. pumilum. Silencing LpNAC48 or LpBBX28 impaired trichome development and reduced trichome density on the outer perianths. We demonstrated that the upstream regulators LpNAC48 and LpBBX28 directly bound to the promoter of the bHLH TF-encoding gene LpGL3-LIKE (LpGL3L) to activate its expression. Moreover, an ABA-responsive element within a 259-bp DNA variation in the LpNAC48 promoter was important for its expression and was bound by the bZIP TF LpbZIP29 during trichome development. This binding activated LpNAC48 expression and contributed to trichome formation. This study provides insights into the role of a small DNA sequence variation in gene expression and trichome traits.
表皮毛在植物抵抗非生物和生物胁迫中起着关键作用，并且它们的发育和特征在不同物种间存在差异。本研究表明，细叶百合的表皮毛在花芽分化期间呈现同步生长，并增强了植株对UV-B辐射和蚜虫感染的适应性。我们鉴定出一个NAC家族转录因子LpNAC48，它在细叶百合表皮毛形成过程中与B-box（BBX）家族转录因子LpBBX28相互作用。沉默LpNAC48或LpBBX28会损害表皮毛发育，并降低外花被片上的表皮毛密度。我们证明，上游调节因子LpNAC48和LpBBX28直接结合到编码bHLH转录因子的基因LpGL3-LIKE（LpGL3L）的启动子上，以激活其表达。此外，LpNAC48启动子中一个259 bp DNA变异内的ABA响应元件对其表达很重要，并且在表皮毛发育过程中被bZIP转录因子LpbZIP29结合。这种结合激活了LpNAC48的表达并有助于表皮毛形成。本研究为一个小DNA序列变异在基因表达和表皮毛性状中的作用提供了见解。
