Broström H, Hellström U, Ziverts I, Obel N, Perlmann P
Vet Immunol Immunopathol. 1985 Jan;8(1-2):47-61. doi: 10.1016/0165-2427(85)90109-6.
In a preceding report we have shown that the lectin Helix pomatia A hemagglutinin (HP) binds to two subpopulations of neuraminidase-treated equine peripheral blood lymphocytes (PBL), constituting about 20% and 75% of PBL, respectively. The aim of the present study was to further characterize these HP+ cells in regard to other surface markers such as receptors for guinea pig erythrocytes (GPR+ cells), membrane-bound immunoglobulins (sIg+ cells), receptors for activated complement (C3R+ cells) and receptors for IgG (Fc alpha R+ cells). This was done by double marker analysis and by lymphocyte fractionation either on columns charged with HP coupled to Sepharose beads or by rosetting with guinea pig erythrocytes. The fractions were also analysed for their proliferative response in the mixed lymphocyte tumor cell interaction (MLTC) assay and to the mitogens leucoagglutinin (La) and concanavalin A (Con A). The results revealed that the majority of GPR+ cells also expressed high avidity receptors for HP, as defined by means of direct immunofluorescence. These cells constituted a subpopulation of GPR+/HP+ cells T cells comprising approximately 20% of PBL. In contrast, about 75% of the HP+ cells in indirect immunofluorescence were GPR-. The fractionation experiments showed that HP+ and GPR+ cells were probably not B cells since they were sIg-. The C3R+ and Fc alpha R+ lymphocytes were heterogeneous in regard to HP receptors but the majority of these cells was also found in the fractions depleted of HP+ and GPR+ lymphocytes. The fractions eluted from HP columns gave a strong proliferative response in MLTC, whereas fractions depleted of HP+ cells responded poorly. However, in contrast to the GPR+-depleted fractions, those enriched in GPR+ lymphocytes responded poorly to the T cell mitogens La and Con A. The mitogenic response of the HP-column fractions to La and to Con A was variable. The results are discussed in relation to HP being a surface marker for a heterogeneous population of equine T cells.
在之前的一份报告中，我们已经表明，凝集素圆口螺A血凝素（HP）与经神经氨酸酶处理的马外周血淋巴细胞（PBL）的两个亚群结合，分别占PBL的约20%和75%。本研究的目的是根据其他表面标志物，如豚鼠红细胞受体（GPR+细胞）、膜结合免疫球蛋白（sIg+细胞）、活化补体受体（C3R+细胞）和IgG受体（FcαR+细胞），进一步表征这些HP+细胞。这是通过双标记分析以及在装有与琼脂糖珠偶联的HP的柱上进行淋巴细胞分级分离或通过与豚鼠红细胞进行花环试验来完成的。还分析了这些分级分离物在混合淋巴细胞肿瘤细胞相互作用（MLTC）试验中以及对有丝分裂原白细胞凝集素（La）和刀豆球蛋白A（Con A）的增殖反应。结果显示，通过直接免疫荧光法定义，大多数GPR+细胞也表达对HP的高亲和力受体。这些细胞构成了GPR+/HP+细胞T细胞亚群，约占PBL的20%。相比之下，间接免疫荧光中约75%的HP+细胞是GPR-。分级分离实验表明，HP+和GPR+细胞可能不是B细胞，因为它们是sIg-。C3R+和FcαR+淋巴细胞在HP受体方面是异质的，但这些细胞中的大多数也存在于HP+和GPR+淋巴细胞耗尽的分级分离物中。从HP柱上洗脱的分级分离物在MLTC中产生强烈的增殖反应，而HP+细胞耗尽的分级分离物反应较差。然而，与GPR+耗尽的分级分离物不同，富含GPR+淋巴细胞的分级分离物对T细胞有丝分裂原La和Con A反应较差。HP柱分级分离物对La和Con A的有丝分裂反应是可变的。结合HP作为马T细胞异质群体的表面标志物这一点对结果进行了讨论。
