There is limited information on the occurrence of and ticks, as well as associated and species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (, and spp.) in Balochistan, Pakistan. A total of 314 ticks were collected from 74 of 117 (63.2%) hosts, including 41 of 74 (55.4%) goats and 33 of 74 (44.5%) sheep. Subsequently, a subset of microscopically identified ticks was subjected to DNA extraction and PCR to amplify 16S rDNA and fragments. Additionally, , and fragments were targeted for spp. and 16S rDNA fragments for both and spp. The 16S rDNA and sequences of demonstrated 100% identity with those of the same species previously reported from Pakistan. The 16S rDNA and sequences of exhibited 99.52 and 100% identities, respectively, with corresponding species reported from China, Kazakhstan, and Turkey. The and fragments associated with . showed 100% identities with reported from Egypt, Italy, Kazakhstan, Kenya, Pakistan, and Senegal. The 16S rDNA sequences of sp. and sp. associated with both . and . exhibited 99.67 and 100% identities with unknown sp. and sp. reported from Morocco and Pakistan, respectively. In the 16S rDNA and phylogenetic trees of ticks, . and . from the current study clustered with their respective species. Similarly, in and phylogenetic trees of , of the present study clustered with the same species, whereas sp. and sp. of this study clustered with undetermined spp. and spp. in the 16S rDNA phylogenetic tree of Anaplasmataceae. Among the DNA samples from the screened ticks, a coinfection rate of , sp., and sp. (2 of 80, 2.5%) was observed in , whereas individual infection rates were noted as follows: (8 of 80, 10%), sp. (5 of 80, 6.3%), and sp. (5 of 80, 6.3%). This study marks the first record of molecular characterization of . and . as well as the detection of associated , sp., and sp. in Balochistan, Pakistan.