Jiao Weijie, Zhou Huihui, Zhang Jun, Yuan Yuan, Wei Junci, Gong Xue, Sun Yuanyuan, Sang Lin, Yu Ming
Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, No.127 Changle West Rd, 710032 Xi'an, Shaanxi, China.
Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, No.169 Changle West Rd, 710032 Xi'an, China.
Biomater Adv. 2025 Apr;169:214171. doi: 10.1016/j.bioadv.2024.214171. Epub 2025 Jan 6.
The objective of this study is to elucidate the sensitizing effect of mesoporous silica nanoparticles (MSNs) on shear wave elastography (SWE) and to investigate the potential application of MSNs as a sensitizer to enhance the sensitivity of SWE in the diagnosis of metabolic-associated steatohepatitis (MASH).
The in vitro gelatin models with varying ratios were assessed using SWE to identify the gelatin ratio that most closely approximates with human liver stiffness. Following the characterization of the dispersion properties of MSNs, in vitro models incorporating MSNs of different particle sizes were developed. The variations in shear wave velocity (SWV) within these models were measured and subjected to statistical analysis using SWE. The biocompatibility of the MSNs was evaluated, and the MSN solution was subsequently administered into a MASH animal model. The sensitizing effect of SWE on rat liver was then analyzed statistically.
The in vitro model demonstrated that MSNs with smaller particle sizes (100 nm and 200 nm) facilitated the propagation of SWV, thereby enhancing the sensitivity of SWE (P < 0.05). Additionally, the cell viability and hemolysis ratio of 100 nm MSNs were superior to those of 200 nm MSNs (P < 0.05). In vivo animal model experiments indicated that 100 nm fluorescence-modified MSNs could penetrate the MASH liver and elevate the liver stiffness value as measured by SWE.
MSNs have the potential to enhance the sensitivity of SWE in the diagnosis of MASH. This approach offers novel insights for improving the efficacy of SWE in clinical diagnostic and therapeutic applications.
本研究的目的是阐明介孔二氧化硅纳米颗粒（MSNs）对剪切波弹性成像（SWE）的增敏作用，并研究MSNs作为增敏剂在提高SWE诊断代谢相关脂肪性肝炎（MASH）敏感性方面的潜在应用。
使用SWE评估不同比例的体外明胶模型，以确定最接近人类肝脏硬度的明胶比例。在表征MSNs的分散特性后，建立了包含不同粒径MSNs的体外模型。测量这些模型内剪切波速度（SWV）的变化，并使用SWE进行统计分析。评估MSNs的生物相容性，随后将MSN溶液注入MASH动物模型。然后对SWE对大鼠肝脏的增敏作用进行统计学分析。
体外模型表明，较小粒径（100 nm和200 nm）的MSNs促进了SWV的传播，从而提高了SWE的敏感性（P < 0.05）。此外，100 nm MSNs的细胞活力和溶血率优于200 nm MSNs（P < 0.05）。体内动物模型实验表明，100 nm荧光修饰的MSNs可以穿透MASH肝脏，并提高SWE测量的肝脏硬度值。
MSNs有潜力提高SWE诊断MASH的敏感性。这种方法为提高SWE在临床诊断和治疗应用中的疗效提供了新的见解。
