Liu Qi, Yu Jiaying, Chen Lucheng, Han Jiaxing, Cai Xiangyu, Hu Shaojun, Chu Xianfeng, Zhang Weijie, Wang Zhifei
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.
Division of Breast Surgery, Department of General Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China.
Talanta. 2025 May 1;286:127478. doi: 10.1016/j.talanta.2024.127478. Epub 2025 Jan 3.
As a serine hydrolase synthesized by the liver, butyrylcholinesterase (BChE) is an important biomarker in the clinical diagnosis of liver diseases. To track BChE activity in drug-induced liver injury, we designed a deep-red BChE-activatable fluorescent probe (CYL-BChE) with hemi-cyanine structure by using a cyclopropyl carbonyl group as a specific recognition moiety. Its near-infrared absorption wavelength (665 nm) and emission wavelength (762 nm) provide excellent tissue penetration capabilities, making it suitable for biological imaging. Additionally, CYL-BChE has a large Stokes shift (97 nm) and a low detection limit (0.96 U/L), effectively distinguishing BChE from the interfering substance acetylcholinesterase (AChE). Besides of detection of endogenous BChE in live cells, the resulting probe has been successfully used to detect BChE expression levels in an acetaminophen-induced (APAP-induced) drug liver injury mouse model. Therefore, this probe is expected to be a valuable biological tool in the detection of BChE activity.
作为一种由肝脏合成的丝氨酸水解酶，丁酰胆碱酯酶（BChE）是肝脏疾病临床诊断中的重要生物标志物。为了追踪药物性肝损伤中的BChE活性，我们设计了一种具有半花青结构的深红色BChE可激活荧光探针（CYL-BChE），以环丙基羰基作为特异性识别基团。其近红外吸收波长（665nm）和发射波长（762nm）具有出色的组织穿透能力，使其适用于生物成像。此外，CYL-BChE具有较大的斯托克斯位移（97nm）和较低的检测限（0.96U/L），能有效区分BChE与干扰物质乙酰胆碱酯酶（AChE）。除了检测活细胞中的内源性BChE外，该探针还成功用于检测对乙酰氨基酚诱导（APAP诱导）的药物性肝损伤小鼠模型中的BChE表达水平。因此，该探针有望成为检测BChE活性的有价值的生物工具。
