Selig Jessica Isabel, Sugimura Yukiharu, Katahira Shintaro, Polidori Marco, Jacobi Laura Alida, Medovoj Olga, Betke Sarah, Barth Mareike, Lichtenberg Artur, Akhyari Payam, Minol Jan-Philipp
Department of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, Germany.
Department of Cardiac Surgery, University of Essen, Hufelandstrasse 55, 45147 Essen, Germany.
Antioxidants (Basel). 2024 Dec 20;13(12):1570. doi: 10.3390/antiox13121570.
Degenerative aortic valve disease (DAVD) is a multifactorial process. We developed an animal model to analyze the isolated, local effect of reactive oxygen species (ROS) on its pathophysiology.
We utilized a photodynamic reaction (PDR) as a source of ROS in the aortic valve by aiming a laser at the aortic valve for 60 min after the administration of a photosensitizer 24 h prior. ROS, laser, and sham groups ( = 7 each) for every observation period (t = 0; t = 8 d; t = 84 d; t = 168 d) were established. The amount of ROS generation; morphological changes; inflammatory, immune, and apoptotic reactions; and hemodynamic changes in the aortic valves were assessed using appropriate histological, immunohistological, immunohistochemical, and echocardiographic methods.
The ROS group displayed an increased amount of ROS ( < 0.01) and increased inflammatory activation of the endothelium ( < 0.05) at t = 0. In the ROS group, aortic valves were calcified ( < 0.05) and the transvalvular gradient was increased ( < 0.01) at t = 168 d.
The small animal model employed here may serve as a platform for analyzing ROS's isolated role in the DAVD context.
退行性主动脉瓣疾病（DAVD）是一个多因素过程。我们建立了一种动物模型，以分析活性氧（ROS）对其病理生理学的独立局部影响。
我们通过在给药光敏剂24小时后用激光照射主动脉瓣60分钟，利用光动力反应（PDR）作为主动脉瓣中ROS的来源。为每个观察期（t = 0；t = 8天；t = 84天；t = 168天）建立了ROS组、激光组和假手术组（每组n = 7）。使用适当的组织学、免疫组织学、免疫化学和超声心动图方法评估主动脉瓣中ROS的生成量、形态变化、炎症、免疫和凋亡反应以及血流动力学变化。
在t = 0时，ROS组的ROS量增加（P < 0.01），内皮的炎症激活增加（P < 0.05）。在t = 168天时，ROS组的主动脉瓣发生钙化（P < 0.05），跨瓣压差增加（P < 0.01）。
这里使用的小动物模型可作为分析ROS在DAVD背景下独立作用的平台。
