Jeon Wook Jong, Kim Yoo Jung, Seo Ju Hui, Yoo Jung Sik, Moon Dong Chan
Division of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of Korea.
Antibiotics (Basel). 2024 Nov 23;13(12):1124. doi: 10.3390/antibiotics13121124.
BACKGROUND/OBJECTIVES: Bloodstream infection by carbapenem-resistant (CRAB) is a serious clinical problem worldwide. To study its clonal relationship and genetic features, we report the draft genome sequence of CRAB strains isolated from human blood in South Korea.
Among strains isolated from patients at nine general hospitals in 2020, 12 CRAB strains of different genotypes were selected. Genomic DNA was sequenced using a combination of Illumina MiSeq and Oxford Nanopore MinION platforms. Antimicrobial susceptibility testing was performed using the disk diffusion method. Antimicrobial resistance and virulence genes were investigated in silico using the Center for Genomic Epidemiology server and the Virulence Factors Database.
The multilocus sequence types of isolates included ST191, ST195, ST357, ST369, ST451, ST469, ST491, ST784, ST862, ST1933, ST2929, and a novel type, ST3326. The predominant sequence type, ST191, demonstrated close genetic relationships with several isolates, including ST469, ST369, ST195, ST784, ST491, and ST3326, with ST3326 classified as a subgroup of ST191. We found 18 antimicrobial resistance genes and one quaternary ammonium compound resistance gene. All examined strains harbored , which is associated with carbapenem resistance. While variations in antibiotic and disinfectant resistance genes were observed, all isolates exhibited similar virulence factors, with the exception of the biofilm and capsule production genes.
This nationwide report of the draft genome sequence of patient-derived strains provides valuable insights into the genomic features associated with clonal relationships and antimicrobial resistance of CRAB in bloodstream infections.
背景/目的：耐碳青霉烯类鲍曼不动杆菌（CRAB）引起的血流感染是全球范围内严重的临床问题。为研究其克隆关系和遗传特征，我们报告了从韩国人血液中分离出的CRAB菌株的基因组草图序列。
在2020年从9家综合医院患者中分离出的菌株中，选择了12株不同基因型的CRAB菌株。使用Illumina MiSeq和Oxford Nanopore MinION平台组合对基因组DNA进行测序。采用纸片扩散法进行药敏试验。利用基因组流行病学中心服务器和毒力因子数据库对耐药性和毒力基因进行了计算机分析。
分离株的多位点序列类型包括ST191、ST195、ST357、ST369、ST451、ST469、ST491、ST784、ST862、ST1933、ST2929，以及一种新型ST3326。主要序列类型ST191与包括ST469、ST369、ST195、ST784、ST491和ST3326在内的多个分离株显示出密切的遗传关系，其中ST3326被归类为ST191的一个亚组。我们发现了18个耐药基因和1个季铵化合物耐药基因。所有检测菌株均携带与碳青霉烯耐药相关的 。虽然观察到抗生素和消毒剂耐药基因存在变异，但除生物膜和荚膜产生基因外，所有分离株均表现出相似的毒力因子。
这份关于患者来源菌株基因组草图序列的全国性报告为血流感染中CRAB的克隆关系和抗菌药物耐药性相关的基因组特征提供了有价值的见解。
