Shu Rundong, Liu Ge, Xu Yunyu, Liu Bojun, Huang Zhi, Wang Hui
Sanya Institute of Nanjing Agricultural University, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Zhengzhou Agricultural Science and Technology Research Institute, Zhengzhou 450015, China.
Antibiotics (Basel). 2024 Nov 29;13(12):1146. doi: 10.3390/antibiotics13121146.
Bile salts possess innate antibacterial properties and can cause significant damage to bacteria. To survive in the mammalian gut, has developed mechanisms to tolerate bile salts; however, the specific mechanisms remain unclear. Transposon library screening revealed that the efflux pump AcrAB is involved in bile salt resistance. and mutants exhibited high sensitivity not only to bile salts but also to SDS and various antibiotics, with a switch-loop, comprising residues G615, F616, A617, and G618, proving to be crucial in this process. A colonization defect of and mutants was demonstrated to be located in the mouse small intestine, where the bile salt concentration is higher compared to the large intestine. Additionally, both and mutants displayed reduced virulence in the model. In conclusion, our results suggest that the Resistance-Nodulation-Cell Division efflux pump serves as a critical determinant in the pathogenesis of through various aspects.
胆盐具有固有的抗菌特性，可对细菌造成显著损害。为了在哺乳动物肠道中生存，[细菌名称]已发展出耐受胆盐的机制；然而，具体机制仍不清楚。转座子文库筛选显示，外排泵AcrAB参与胆盐抗性。[细菌名称]突变体不仅对胆盐，而且对SDS和各种抗生素都表现出高敏感性，由G615、F616、A617和G618残基组成的开关环在这一过程中被证明至关重要。[细菌名称]突变体的定殖缺陷被证明位于小鼠小肠，与大肠相比，小肠中的胆盐浓度更高。此外，[细菌名称]突变体在[感染模型名称]模型中均表现出毒力降低。总之，我们的结果表明，耐药-固氮-细胞分裂外排泵通过多个方面在[细菌名称]的发病机制中起关键作用。
