（螯虾亚目：螯虾科）线粒体基因组的特征及其系统发育意义

Characterization of the Mitochondrial Genome of (Astacidea: Cambaridae) and Its Phylogenetic Implications.

作者信息

Liu Xuewei, Li Ben, Yang Yan, Zhang Jun, Hu Chunbo, Zhang Yuxi, Zhou Jiawang, Liu Yinlong, Qiu Hongyu, Wang Chunren, Gao Junfeng

机构信息

Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Animal Disease Prevention and Control Center in Huanan County, Jiamusi 154400, China.

出版信息

Genes (Basel). 2024 Dec 8;15(12):1578. doi: 10.3390/genes15121578.

DOI:10.3390/genes15121578

PMID:39766845

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11675430/

Abstract

BACKGROUND

is an endangered freshwater crayfish in China, belonging to the genus Cambaroides, that can act as a complementary host for paragonimus. The objective of this study was to examine the complete mitochondrial genome characteristics and their evolutionary relationships within the Astacidea.

METHODS

The analysis of gene rearrangements and evolutionary relationships was conducted through the sequencing of the mitochondrial genome of .

RESULTS

mitochondrial genome length was 15,572, comprising thirteen PCGs, two rRNAs, 22 tRNAs, and one d-loop region of . The mitochondrial genome of exhibits an A + T content of 69.61% and a G + C content of 30.39%. Among the thirteen PCGs, cytb, nad3, and nad6 have a start codon of ATT, while the other ten PCGs have ATC, ATA, and ATG start codons. All 22 tRNA genes displayed a typical cloverleaf secondary structure. Gene rearrangement analysis showed that seven gene arrangements were identified based on PCGs in the infraorder Astacidea, with type I being the most common.

CONCLUSIONS

The relationship between the American Cambaridae is closer to Astacidae than the Asian Cambaridae. The present study provides a theoretical basis for further discussions of developmental relationships in the infraorder Astacidea.

摘要

背景

是中国一种濒危淡水小龙虾，属于蝲蛄属，可作为并殖吸虫的补充宿主。本研究的目的是研究其线粒体基因组的完整特征及其在螯虾下目中的进化关系。

方法

通过对的线粒体基因组进行测序，分析基因重排和进化关系。

结果

线粒体基因组长度为15572，包括13个蛋白质编码基因（PCGs）、2个核糖体RNA（rRNAs）、22个转运RNA（tRNAs）和1个d-loop区域。的线粒体基因组A+T含量为69.61%，G+C含量为30.39%。在13个PCGs中，细胞色素b（cytb）、烟酰胺腺嘌呤二核苷酸脱氢酶亚基3（nad3）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基6（nad6）的起始密码子为ATT，而其他10个PCGs的起始密码子为ATC、ATA和ATG。所有22个tRNA基因均呈现典型的三叶草二级结构。基因重排分析表明，基于螯虾下目PCGs鉴定出7种基因排列类型，其中I型最为常见。