Dekleva Milica, Djuric Tamara, Djordjevic Ana, Soldatovic Ivan, Stankovic Aleksandra, Suzic Lazic Jelena, Zivkovic Maja
Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Laboratory for Radiobiology and Molecular Genetics, VINČA Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Biomedicines. 2024 Nov 21;12(12):2661. doi: 10.3390/biomedicines12122661.
Galectin-3 (gal-3) is upregulated in remodeling, and failing myocardium and gal-3 levels are increased in hypertrophy, fibrosis and inflammation. The aim of this study was to investigate the potential role of sex-related differences in the following: risk factors, left ventricular (LV) structural and functional changes, coronary angiography, expression of the gal-3 encoding gene and plasma gal-3 levels in heart failure (HF). : This prospective study included 137 men and 44 women with first MI who underwent Doppler echocardiography within 2-4 days of MI and after 6 months. Relative mRNA expression in peripheral blood mononuclear cells (PBMCs) was detected using TaqMan technology. Plasma gal-3 concentration was determined by ELISA method. : In the acute phase of MI, LV end-diastolic and end-systolic volume indexes (LVEDVI and LVESVI) were significantly lower in women compared to men (58.2 ± 13.1 vs. 46.3 ± 11.1, < 0.001; 33.7 ± 9.5 vs. 27.0 ± 9.2, < 0.001, respectively). The incidence of LV hypertrophy (LVH) and HF was significantly higher in women compared to men (70.0% vs. 44.6%, = 0.03; 37.5% vs.19.5%, = 0.02, respectively). There was a significant correlation between the grade of LV diastolic dysfunction (LVDD) and plasma gal-3 levels ( < 0.001). The relative expression of mRNA in PBMCs was higher in females (fold induction = 1.326, S.E. range = 0.748-2.587, = 0.007). Plasma gal-3 levels were higher in women compared to men (44.66 ± 28.04 vs. 16.30 ± 12.68, < 0.001) and higher in patients with HF than in patients without HF (31.14 ± 27.09 vs.21.39 ± 18.17, = 0.025). : Gender-specific factors such as LVH, LVDD, mRNA expression and plasma gal-3 levels may explain the increased incidence of HF in women. The differences in the model and determinants of HF between men and women may be relevant for further therapeutic strategies including the inhibition of gal-3.
半乳糖凝集素-3（gal-3）在心肌重塑和衰竭心肌中表达上调，且在心肌肥厚、纤维化和炎症时gal-3水平升高。本研究旨在探讨性别差异在以下方面的潜在作用：心力衰竭（HF）的危险因素、左心室（LV）结构和功能变化、冠状动脉造影、gal-3编码基因的表达以及血浆gal-3水平。：这项前瞻性研究纳入了137名男性和44名首次发生心肌梗死（MI）的女性，他们在MI后2 - 4天及6个月后接受了多普勒超声心动图检查。采用TaqMan技术检测外周血单个核细胞（PBMCs）中的相对mRNA表达。采用ELISA法测定血浆gal-3浓度。：在MI急性期，女性的左心室舒张末期和收缩末期容积指数（LVEDVI和LVESVI）显著低于男性（分别为58.2±13.1对46.3±11.1，<0.001；33.7±9.5对27.0±9.2，<0.001）。女性左心室肥厚（LVH）和HF的发生率显著高于男性（分别为70.0%对44.6%，P = 0.03；37.5%对19.5%，P = 0.02）。左心室舒张功能障碍（LVDD）分级与血浆gal-3水平之间存在显著相关性（<0.001）。女性PBMCs中mRNA的相对表达较高（诱导倍数 = 1.326，标准误范围 = 0.748 - 2.587，P = 0.007）。女性的血浆gal-3水平高于男性（44.66±28.04对16.30±12.68，<0.001），且HF患者高于非HF患者（31.14±27.09对21.39±18.17，P = 0.025）。：诸如LVH、LVDD、mRNA表达和血浆gal-3水平等性别特异性因素可能解释了女性HF发生率增加的原因。男性和女性之间HF模型和决定因素的差异可能与包括抑制gal-3在内的进一步治疗策略相关。
