Panahipour Layla, Micucci Chiara, Gruber Reinhard
Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria.
Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.
Cells. 2024 Dec 13;13(24):2062. doi: 10.3390/cells13242062.
THP1 and U937 are monocytic cell lines that are common bioassays to reflect monocyte and macrophage activities in inflammation research. However, THP-1 is a human monocytic leukemia cell line, and U937 originates from pleural effusion of histiocytic lymphoma; thus, even though they serve as bioassay in inflammation research, their response to agonists is not identical. Consequently, there has yet to be a consensus about the panel of strongly regulated genes in THP1 and U937 cells representing the inflammatory response to LPS and IFNG. Therefore, we have performed an RNAseq of THP1 and U937 exposed to LPS and IFNG to identify the most sensitive genes and the unique properties of each individual cell line. When applying a highly stringent threshold, we could identify 43, 8 up and 94, 103 down-regulated genes in THP1 and U937 cells, respectively. In THP1 cells, among the most strongly up-regulated genes are , , , , , , and . In U937 cells, the strongest up-regulated genes include , , , , , , , , , , , and . Even though THP1 is considerably less responsive than U937, there are genes commonly upregulated by LPS and IFNG, including the , , , , , , as well as , , , , and genes of prostaglandin synthesis and . Downregulated genes are limited to and . This head-to-head comparison revealed that THP1 is less responsive than U937 cells to LPS and IFNG and identified a panel of highly regulated genes that can be applied in bioassays in inflammation research. Our data further propose bulk RNAseq as a standard method in bioassay research.
THP1和U937是单核细胞系，在炎症研究中是反映单核细胞和巨噬细胞活性的常见生物测定法。然而，THP-1是一种人单核细胞白血病细胞系，U937起源于组织细胞淋巴瘤的胸腔积液；因此，尽管它们在炎症研究中用作生物测定法，但它们对激动剂的反应并不相同。因此，关于THP1和U937细胞中代表对LPS和IFNG炎症反应的强调控基因面板，尚未达成共识。因此，我们对暴露于LPS和IFNG的THP1和U937进行了RNA测序，以确定最敏感的基因以及每个细胞系的独特特性。当应用高度严格的阈值时，我们分别在THP1和U937细胞中鉴定出43个上调基因和8个下调基因，以及94个上调基因和103个下调基因。在THP1细胞中，上调最强烈的基因包括 、 、 、 、 、 和 。在U937细胞中，上调最强烈的基因包括 、 、 、 、 、 、 、 、 、 、 和 。尽管THP1的反应性比U937低得多，但仍有一些基因被LPS和IFNG共同上调，包括 、 、 、 、 、 以及前列腺素合成的 、 、 、 和 基因。下调基因仅限于 和 。这种直接比较表明，THP1细胞对LPS和IFNG的反应性低于U937细胞，并鉴定出一组可用于炎症研究生物测定法的高度调控基因。我们的数据进一步提出将批量RNA测序作为生物测定研究的标准方法。
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