Heikal Yasmin M, Shweqa Nada S, Abdelmigid Hala M, Alyamani Amal A, Soliman Hoda M, El-Naggar Noura El-Ahmady
Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
Department of Biotechnology, College of Science, Taif University, Taif 21944, Saudi Arabia.
Life (Basel). 2024 Nov 27;14(12):1560. doi: 10.3390/life14121560.
This study investigated the biosynthesis, statistical optimization, characterization, and biocontrol activity of silver nanoparticles (AgNPs) produced by newly isolated sp. The strain TA-3N was identified based on the ITS gene sequence, together with its phenotypic characteristics (GenBank accession number: OM321439). The color change from light yellow to brown after the incubation period indicates AgNPs biosynthesis. The UV spectrum revealed a single peak with the maximum absorption at 453 nm, indicating that produces AgNPs effectively. A Rotatable Central Composite Design (RCCD) was used to optimize the biosynthesis of AgNPs using the aqueous mycelial-free filtrate of . The optimal conditions for maximum AgNPs biosynthesis (156.02 µg/mL) were predicted theoretically using the desirability function tool and verified experimentally. The highest biosynthetic produced AgNPs by reached 160.3 µg/mL using AgNO concentration of 2 mM/mL, initial pH level of 6, incubation time of 60 h, and biomass weight of 6 g/100 mL water. SEM and TEM imaging revealed uniform spherical shape particles that varied in size between 8.17 and 17.74 nm. The synthesized AgNPs have a Zeta potential value of -9.51 mV. FTIR analysis provided insights into the surface composition of AgNPs, identifying various functional groups such as N-H, -OH, C-H, C=O, and the amide I bond in proteins. Cytotoxicity and genotoxicity assays demonstrated that AgNPs in combination with can mitigate the toxic effects of on barley. This intervention markedly enhanced cell division rates and decreased chromosomal irregularities. The results indicate that AgNPs synthesized by show the potential as an eco-friendly and efficient method for controlling plant diseases. Further studies are necessary to investigate their possible use in the agricultural sector.
本研究调查了新分离的菌株所产生的银纳米颗粒（AgNPs）的生物合成、统计优化、表征及生物防治活性。基于ITS基因序列及其表型特征鉴定出菌株TA - 3N（GenBank登录号：OM321439）。培养期后颜色从浅黄色变为棕色表明AgNPs的生物合成。紫外光谱显示在453 nm处有一个最大吸收的单峰，表明该菌株能有效产生AgNPs。采用旋转中心复合设计（RCCD），利用该菌株的无菌丝体水滤液优化AgNPs的生物合成。使用期望函数工具从理论上预测了最大AgNPs生物合成（156.02 µg/mL）的最佳条件，并进行了实验验证。当硝酸银浓度为2 mM/mL、初始pH值为6、培养时间为60 h以及生物量为6 g/100 mL水时，该菌株产生的AgNPs生物合成量最高达到160.3 µg/mL。扫描电子显微镜（SEM）和透射电子显微镜（TEM）成像显示颗粒呈均匀球形，尺寸在8.17至17.74 nm之间变化。合成的AgNPs的Zeta电位值为 - 9.51 mV。傅里叶变换红外光谱（FTIR）分析揭示了AgNPs的表面组成，确定了各种官能团，如N - H、 - OH、C - H、C = O以及蛋白质中的酰胺I键。细胞毒性和遗传毒性测定表明，AgNPs与该菌株联合使用可减轻该菌株对大麦的毒性作用。这种干预显著提高了细胞分裂率并减少了染色体异常。结果表明，该菌株合成的AgNPs显示出作为一种环保且高效的植物病害防治方法的潜力。有必要进一步研究其在农业领域的可能用途。
