Sun Zhidan, Ding Chenglong, Wang Yuhan, Lu Tingting, Song Wencheng
Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
College of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
Int J Mol Sci. 2024 Dec 10;25(24):13262. doi: 10.3390/ijms252413262.
Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) is an innovative cancer treatment method that has received considerable scientific attention. The objective of this study is to evaluate the effects of PAM on the anti-tumor characteristics of non-small cell lung cancer (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. The effects of PAM treatment on the proliferative and migratory capabilities of A549 cells in 2D and 3D cultures were assessed using MTT, migration, invasion assays, and cell cycle, respectively. The study also investigated the impact of PAM treatment on the changes in the content of intracellular and extracellular reactive species and analyzed protein expression using the Western Blot method. PAM treatment inhibited the viability, migration, and invasion abilities of A549 cells in both 2D and 3D cultures, suppressed the epithelial-mesenchymal transition (EMT) process, and downregulated the expression of the RAS/ERK signaling pathway, which effectively inhibited tumor spheroid formation. Additionally, the effect of PAM on A549 cells was mediated through ROS-induced oxidative reactions, and PAM treatment exhibited greater cytotoxicity in 2D culture compared to 3D culture. As compared to 2D, the 3D cell culture model provides a viable in vitro cell model for studying the mechanisms of PAM treatment in lung cancer. PAM represents an effective new treatment for NSCLC.
肺癌是全球最常见的恶性肿瘤类型。等离子体激活介质（PAM）是一种创新的癌症治疗方法，已受到相当多的科学关注。本研究的目的是评估PAM在二维（2D）和三维（3D）培养中对非小细胞肺癌（NSCLC）细胞抗肿瘤特性的影响。分别使用MTT、迁移、侵袭试验和细胞周期评估PAM处理对2D和3D培养中A549细胞增殖和迁移能力的影响。该研究还调查了PAM处理对细胞内和细胞外活性物质含量变化的影响，并使用蛋白质印迹法分析蛋白质表达。PAM处理在2D和3D培养中均抑制了A549细胞的活力、迁移和侵袭能力，抑制了上皮-间质转化（EMT）过程，并下调了RAS/ERK信号通路的表达，从而有效抑制了肿瘤球体的形成。此外，PAM对A549细胞的作用是通过ROS诱导的氧化反应介导的，与3D培养相比，PAM处理在2D培养中表现出更大的细胞毒性。与2D相比，3D细胞培养模型为研究PAM治疗肺癌的机制提供了一种可行的体外细胞模型。PAM是一种治疗NSCLC的有效新方法。
