Chen Sa, Li Shuyu, Qian Shiyuan, Xing Jiale, Liao Jingjing, Guo Zhifu
Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110161, China.
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Int J Mol Sci. 2024 Dec 11;25(24):13304. doi: 10.3390/ijms252413304.
Stress on the Endoplasmic reticulum (ER) can severely disrupt cellular function by impairing protein folding and post-translational modifications, thereby leading to the accumulation of poor-quality proteins. However, research on its impact on photosynthesis remains limited. In this study, we investigated the impact of ER stress on the photosynthetic efficiency of Chlamydomonas reinhardtii using pharmacological inducers, tunicamycin (TM) and brefeldin A (BFA), which specifically target the ER. Our measurements of photosynthetic parameters showed that these ER stress-inducing compounds caused a significant decline in photosynthetic efficiency. A proteomic analysis confirmed that TM and BFA effectively induce ER stress, as evidenced by the upregulation of ER stress-related proteins. Furthermore, we observed a widespread downregulation of photosynthesis-related proteins, which is consistent with the results obtained from our measurements of photosynthetic parameters. These findings suggest that the stress on ER has a profound impact on chloroplast function, disrupting photosynthetic processes. This study highlights the critical interdependence between the ER and chloroplasts, and it underscores the broader implications of ER stress on the cellular metabolism and energy efficiency of photosynthetic organisms.
内质网（ER）应激可通过损害蛋白质折叠和翻译后修饰严重扰乱细胞功能，从而导致劣质蛋白质的积累。然而，关于其对光合作用影响的研究仍然有限。在本研究中，我们使用特异性作用于内质网的药理学诱导剂衣霉素（TM）和布雷菲德菌素A（BFA），研究了内质网应激对莱茵衣藻光合效率的影响。我们对光合参数的测量表明，这些内质网应激诱导化合物导致光合效率显著下降。蛋白质组学分析证实，TM和BFA有效诱导内质网应激，内质网应激相关蛋白的上调证明了这一点。此外，我们观察到光合作用相关蛋白普遍下调，这与我们对光合参数测量的结果一致。这些发现表明，内质网应激对叶绿体功能有深远影响，扰乱光合过程。本研究突出了内质网与叶绿体之间的关键相互依存关系，并强调了内质网应激对光合生物细胞代谢和能量效率的更广泛影响。
