Hosseini Samira, Diegelmann Julia, Folwaczny Matthias, Frasheri Iris, Wichelhaus Andrea, Sabbagh Hisham, Seidel Corrina, Baumert Uwe, Janjic Rankovic Mila
Department of Orthodontics and Dentofacial Orthopedics, LMU University Hospital, LMU Munich, 80336 Munich, Germany.
Department of Conservative Dentistry and Periodontology, LMU University Hospital, LMU Munich, 80336 Munich, Germany.
Int J Mol Sci. 2024 Dec 17;25(24):13513. doi: 10.3390/ijms252413513.
Oxidative stress (OS) is a common feature of many inflammatory diseases, oral pathologies, and aging processes. The impact of OS on periodontal ligament cells (PDLCs) in relation to oral pathologies, including periodontal diseases, has been investigated in different studies. However, its impact on orthodontic tooth movement (OTM) remains poorly understood. This study used an in vitro model with human PDLCs previously exposed to HO to investigate the effects of OS under a static compressive force which simulated the conditions of OTM. Human PDLCs were treated with varying concentrations of HO to identify sub-lethal doses that affected viability minimally. To mimic compromised conditions resembling OTM under OS, the cells were pretreated with the selected HO concentrations for 24 h. Using an in vitro loading model, a static compressive force (2 g/cm) was applied for an additional 24 h. The cell viability, proliferation, and cytotoxicity were evaluated using live/dead and resazurin assays. Apoptosis induction was assessed based on caspase-3/7 activity. The gene expression related to bone remodeling (, /, ), inflammation (, /, /), apoptosis (, ), and autophagy (/, ) was analyzed using RT-qPCR. This study suggests an altering effect of previous OS exposure on static-compression-related mechanosensing. Further research is needed to fully elucidate these mechanisms.
氧化应激（OS）是许多炎症性疾病、口腔疾病和衰老过程的共同特征。关于OS对包括牙周疾病在内的口腔疾病中牙周膜细胞（PDLCs）的影响，已有不同研究进行了探讨。然而，其对正畸牙移动（OTM）的影响仍知之甚少。本研究使用了一个体外模型，其中人类PDLCs预先暴露于HO，以研究在模拟OTM条件的静态压缩力下OS的影响。用不同浓度的HO处理人类PDLCs，以确定对细胞活力影响最小的亚致死剂量。为模拟OS下类似于OTM的受损条件，将细胞用选定的HO浓度预处理24小时。使用体外加载模型，再施加24小时的静态压缩力（2 g/cm）。使用活/死和刃天青测定法评估细胞活力、增殖和细胞毒性。基于半胱天冬酶-3/7活性评估凋亡诱导情况。使用RT-qPCR分析与骨重塑（、/、）、炎症（、/、/）、凋亡（）和自噬（/、）相关的基因表达。本研究表明，先前的OS暴露对静态压缩相关的机械传感有改变作用。需要进一步研究以充分阐明这些机制。
