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金属羧肽酶抑制剂家族的全基因组分析表明，其影响[植物名称]的根系发育和托烷生物碱合成。 （注：原文中“that”后缺少具体内容，翻译时根据语境补充了“其”指代不明的部分）

Genome-Wide Analysis of the Metallocarboxypeptidase Inhibitor Family Reveals That Affects Root Development and Tropane Alkaloid Production in .

作者信息

Yang Shengyu, Wang Yi, Wan Shiyu, Zhang Can, Liao Siyuan, Chen Min, Lan Xiaozhong, Liao Zhihua, Zeng Lingjiang

机构信息

Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China.

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.

出版信息

Int J Mol Sci. 2024 Dec 23;25(24):13729. doi: 10.3390/ijms252413729.

DOI:10.3390/ijms252413729
PMID:39769489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11676366/
Abstract

is a medicinal plant and an important source for the commercial production of tropane alkaloids (TAs), such as scopolamine and hyoscyamine, which are used clinically for their anticholinergic properties. In this study, we identified 16 metallocarboxypeptidase inhibitor (MCPI) genes from (), which are grouped into three subgroups based on phylogenetic relationships and are distributed across 10 chromosomes. Promoter analysis showed that most elements were related to defense and stress responses, such as drought, low-temperature, ABA (abscisic acid), GA (gibberellin), auxin, light and MeJA responsiveness. A gene encoding a putative metallocarboxypeptidase inhibitor () is cloned from and characterized. shows similar tissue expression pattern to TA biosynthesis genes such as , , , etc., with exclusive expression in the roots. When is silenced by virus-induced gene silencing (VIGS), the root growth is markedly inhibited and the production of hyoscyamine and scopolamine is significantly reduced. Our findings indicate a positive role of AbMCPI8 in root development, which could positively affect TA production in .

摘要

是一种药用植物，也是商业生产托烷生物碱（TAs）的重要来源，如东莨菪碱和莨菪碱，它们因其抗胆碱能特性而被临床使用。在本研究中，我们从（）中鉴定出16个金属羧肽酶抑制剂（MCPI）基因，根据系统发育关系将它们分为三个亚组，并分布在10条染色体上。启动子分析表明，大多数元件与防御和应激反应有关，如干旱、低温、脱落酸（ABA）、赤霉素（GA）、生长素、光和茉莉酸甲酯反应。从克隆了一个编码假定金属羧肽酶抑制剂（）的基因并进行了表征。与、、等TA生物合成基因显示出相似的组织表达模式，在根中特异性表达。当通过病毒诱导的基因沉默（VIGS）使沉默时，根生长受到明显抑制，莨菪碱和东莨菪碱的产量显著降低。我们的研究结果表明AbMCPI8在根发育中具有积极作用，这可能会对中的TA产生产生积极影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/737c42013d4f/ijms-25-13729-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/3551ba4abb33/ijms-25-13729-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/079ebc0fd6b0/ijms-25-13729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/5ab0c5cf5559/ijms-25-13729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/1e1f72880bfb/ijms-25-13729-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/a19f0046c86b/ijms-25-13729-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/8308c4b007c7/ijms-25-13729-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/737c42013d4f/ijms-25-13729-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/3551ba4abb33/ijms-25-13729-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/3ae86b38ccfb/ijms-25-13729-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/079ebc0fd6b0/ijms-25-13729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/5ab0c5cf5559/ijms-25-13729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/1e1f72880bfb/ijms-25-13729-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/a19f0046c86b/ijms-25-13729-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/8308c4b007c7/ijms-25-13729-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e4b/11676366/737c42013d4f/ijms-25-13729-g008.jpg

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