Zhang Yue, Zhao Ruiwen, Jing Tingting, Lin Sixi, Ding Xiaolei
Co-Innovation Centre for Sustainable Forestry in Southern China, Forestry and Grassland, College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China.
Int J Mol Sci. 2024 Dec 23;25(24):13732. doi: 10.3390/ijms252413732.
is one of the most destructive quarantine pests, causing irreversible damage to pine trees. However, the unexpected identification of pine wilt disease in Northern China indicates that can survive under low temperatures. In this study, we analyzed the reproductivity variations among 18 different isolates, and SC13 was identified to have excellent low temperature resistance. Subsequent molecular analysis of SC13 indicated its distinct gene expression under low temperatures. The epidermal growth factor, nematode cuticle collagen and G-protein-coupled receptor genes with environmental adaptation functions were demonstrated to be differentially expressed under low temperatures. Meanwhile, morphological observations also indicated that SC13 contained significantly more lipid drops in low-temperature treatments. Generally, the identification of representative isolates will facilitate relevant studies in the future, and the discovery of the gene expression and morphological changes of under low temperatures could expand the current understanding of the environmental adaption abilities of such invasive nematodes.
是最具破坏性的检疫性害虫之一，会对松树造成不可逆转的损害。然而，在中国北方意外发现松材线虫病表明其能够在低温下存活。在本研究中，我们分析了18种不同分离株的繁殖力差异，鉴定出SC13具有优异的低温抗性。随后对SC13的分子分析表明其在低温下有独特的基因表达。具有环境适应功能的表皮生长因子、线虫表皮胶原蛋白和G蛋白偶联受体基因在低温下被证明有差异表达。同时，形态学观察也表明，在低温处理下SC13含有明显更多的脂滴。总体而言，代表性分离株的鉴定将有助于未来的相关研究，而松材线虫在低温下基因表达和形态变化的发现可能会扩展目前对这种入侵线虫环境适应能力的理解。
