Degradi Luca, Tava Valeria, Esposto Maria Carmela, Prigitano Anna, Bulgari Daniela, Kunova Andrea, Saracchi Marco, Cortesi Paolo, Pasquali Matias
Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133 Milan, Italy.
Department of Imaging & Pathology, KU Leuven, RK-Herestraat 49, 3000 Leuven, Belgium.
Pathogens. 2024 Dec 3;13(12):1062. doi: 10.3390/pathogens13121062.
is an important plant pathogen in maize and other cereals that is seldom detected as the cause of human fusariosis. Here, we provide the analysis of the available diversity of sequenced worldwide and report the first two genome assemblies and annotations (including mitochondrial DNA) of from clinical settings. 05-0160 (IUM05-0160) and 09-1037 (IUM09-1037) strains were obtained from the bone marrow and blood of two immunocompromised patients, respectively. The phylogenomic analysis confirmed the species identity of our two strains. Comparative genomic analyses among the reannotated genomes ( = 46) did not lead to the identification of unique genes specific to the clinical samples. Two subgroups in the clade were also identified and confirmed by a mitochondrial diversity study. Clinical strains ( = 4) were positioned in the multigene phylogenetic tree without any correlation between the host and the tree branches, grouping with plant-derived strains. To investigate the existence of a potential fitness advantage of our two clinical strains, we compared demethylase inhibitor fungicides susceptibility against the reference 7600, showing, on average, lower susceptibility to agricultural and medical-used antifungals. A significant reduction in susceptibility was observed for itraconazole and tetraconazole, which might be explained by structural changes in CYP51A and CYP51C sequences. By providing the first two annotated genomes of from clinical settings comprehensive of their mitogenomes, this study can serve as a base for exploring the fitness and adaptation capacities of infecting different kingdoms.
是玉米和其他谷物中的一种重要植物病原体，很少被检测为人类镰刀菌病的病因。在这里，我们对全球测序的可用多样性进行了分析，并报告了来自临床环境的该菌的前两个基因组组装和注释（包括线粒体DNA）。05 - 0160（IUM05 - 0160）和09 - 1037（IUM09 - 1037）菌株分别从两名免疫功能低下患者的骨髓和血液中获得。系统基因组分析证实了我们两个菌株的物种身份。对重新注释的该菌基因组（n = 46）进行的比较基因组分析未发现临床样本特有的独特基因。通过线粒体多样性研究还鉴定并确认了该菌进化枝中的两个亚组。临床菌株（n = 4）在多基因系统发育树中的定位与宿主和树分支之间没有任何相关性，与植物来源的菌株聚在一起。为了研究我们两个临床菌株是否存在潜在的适应性优势，我们将脱甲基酶抑制剂杀菌剂敏感性与参考菌株7600进行了比较，结果显示，平均而言，对农业和医疗用抗真菌剂的敏感性较低。观察到伊曲康唑和四氟康唑的敏感性显著降低，这可能由CYP51A和CYP51C序列的结构变化来解释。通过提供来自临床环境的该菌的前两个注释基因组及其有丝分裂基因组，本研究可为探索该菌感染不同界的适应性和适应能力奠定基础。
