Cucu Dana
Department of Anatomy, Biophysics and Physiology, Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095 Bucharest, Romania.
Research Institute of the University of Bucharest (ICUB), University of Bucharest, 90-92 Sos. Panduri, 050663 Bucharest, Romania.
Pharmaceuticals (Basel). 2024 Dec 9;17(12):1657. doi: 10.3390/ph17121657.
The expression of the transient receptor potential 1 (TRPA1) gene is increased in many solid tumours, and its function relates to inflammation, oxidative stress or the presence of toxic substances. However, little is known about the correlation of clinical parameters with patients' cancer stages, metastases and the degree of tumour infiltration by immune cells. We performed a bioinformatic analysis, using databases and public resources to investigate TRPA1 for many available samples. We used samples from the TCGA project and quantified the mRNA expression and survival analysis using TIMER, TIMER.2 and GEPIA. To analyse hypermethylation, a more extensive database was available from the UALCAN website. We show that the TRPA1 gene is hypermethylated in many cancers. The high expression of TRPA1 is correlated with a better prognosis for several cancer types and correlates with cancer stage and metastasis, while in others the TRPA1 is pro-oncogenic. We also report the effect of TRPA1 expression in immune infiltrating cells. Moreover, the expression is linked to genes essential for inflammation, oxidative stress and cellular motility processes. Our study brings new insights into the regulation of TRPA1 expression in different tumours based on analysis provided by public databases, opening the possibility to further investigate the protein as a putative target for cancer.
瞬时受体电位1（TRPA1）基因在许多实体瘤中的表达增加，其功能与炎症、氧化应激或有毒物质的存在有关。然而，关于临床参数与患者癌症分期、转移以及免疫细胞肿瘤浸润程度之间的相关性知之甚少。我们进行了一项生物信息学分析，利用数据库和公共资源对许多可用样本中的TRPA1进行研究。我们使用了来自癌症基因组图谱（TCGA）项目的样本，并使用TIMER、TIMER.2和GEPIA对mRNA表达和生存分析进行了量化。为了分析高甲基化，可从UALCAN网站获得更广泛的数据库。我们发现TRPA1基因在许多癌症中发生了高甲基化。TRPA1的高表达与几种癌症类型的较好预后相关，且与癌症分期和转移相关，而在其他癌症中TRPA1具有促癌作用。我们还报告了TRPA1表达在免疫浸润细胞中的作用。此外，该表达与炎症、氧化应激和细胞运动过程所必需的基因有关。我们的研究基于公共数据库提供的分析，为不同肿瘤中TRPA1表达的调控带来了新的见解，为进一步研究该蛋白作为癌症的潜在靶点开辟了可能性。
