Li Fei, Lu Yuntong, Xi Kuanling, Li Yuke, Chen Xiaoyan, Wang Puchang, Huang Xiaolong
School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Microorganisms. 2024 Nov 22;12(12):2392. doi: 10.3390/microorganisms12122392.
Recent advancements in the field of plant-pathogen interactions have spotlighted the role of extracellular vesicles (EVs) as pivotal mediators of cross-kingdom communication, offering new vistas for enhancing crop protection strategies. EVs are instrumental in the transport of small regulatory RNAs (sRNAs) and other bioactive molecules across species boundaries, thus playing a critical role in the molecular warfare between plants and pathogens. This review elucidates the sophisticated mechanisms by which plants utilize EVs to dispatch sRNAs that silence pathogenic genes, fortifying defenses against microbial threats. Highlighting both eukaryotic and prokaryotic systems, this review delves into the biogenesis, isolation, and functional roles of EVs, illustrating their importance not only in fundamental biological processes but also in potential therapeutic applications. Recent studies have illuminated the significant role of EVs in facilitating communication between plants and pathogens, highlighting their potential in host-defense mechanisms. However, despite these advancements, challenges remain in the efficient isolation and characterization of plant-derived EVs. Overcoming these challenges is critical for fully harnessing their potential in developing next-generation crop protection strategies. This review proposes innovative strategies for utilizing RNA-based interventions delivered via EVs to bolster plant resilience against diseases. By integrating the latest scientific findings with practical applications in agriculture, this review aims to enhance the connection between fundamental plant biology and the development of innovative crop management technologies.
植物 - 病原体相互作用领域的最新进展突出了细胞外囊泡（EVs）作为跨界通信关键介质的作用，为加强作物保护策略提供了新的视角。细胞外囊泡有助于小调节RNA（sRNAs）和其他生物活性分子跨物种边界运输，因此在植物与病原体之间的分子对抗中发挥着关键作用。本综述阐明了植物利用细胞外囊泡传递使致病基因沉默的小RNA以加强对微生物威胁防御的复杂机制。本综述突出了真核和原核系统，深入探讨了细胞外囊泡的生物发生、分离及其功能作用，阐述了它们不仅在基础生物学过程中而且在潜在治疗应用中的重要性。最近的研究阐明了细胞外囊泡在促进植物与病原体之间通信方面的重要作用，突出了它们在宿主防御机制中的潜力。然而，尽管有这些进展，在高效分离和鉴定植物来源的细胞外囊泡方面仍然存在挑战。克服这些挑战对于充分利用它们在开发下一代作物保护策略中的潜力至关重要。本综述提出了利用通过细胞外囊泡传递的基于RNA的干预措施来增强植物抗病能力的创新策略。通过将最新科学发现与农业实际应用相结合，本综述旨在加强基础植物生物学与创新作物管理技术开发之间的联系。
