Kondakala Sandeep, Yoon Sunghyun, Daddy Gaoh Soumana, Kweon Ohgew, Kim Seong-Jae, Hart Mark E
Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Microorganisms. 2024 Nov 26;12(12):2432. doi: 10.3390/microorganisms12122432.
The interaction between and strains FRI-1169 and MN8, two original isolated strains from menstrual toxic shock syndrome (mTSS) cases, is a key focus for developing non-antibiotic strategies to control -related infections. While the antagonistic effects of species on through mechanisms like organic acid and bacteriocin production are known, the molecular dynamics of these interactions remain underexplored. This study employs a proteomic approach to analyze the interactions between WCFS1 and strains, FRI-1169 and MN8, during co-culture. We profiled differentially expressed proteins (DEPs) found in the spent media and cytosols of both bacteria, revealing distinct directional and strain-specific responses. The findings demonstrate that exerts a more pronounced effect on , with more DEPs and upregulated proteins, while showed fewer DEPs and more downregulated proteins. These strain-specific interactions highlight the complex metabolic and regulatory adjustments between these bacterial species. This research provides valuable insights into the molecular mechanisms of antagonism and underscores the potential of proteomic analysis as a powerful tool for studying bacterial dynamics in co-culture systems.
来自月经毒性休克综合征（mTSS）病例的两株原始分离菌株FRI - 1169和MN8之间的相互作用，是开发控制相关感染的非抗生素策略的关键重点。虽然已知某些物种通过产生有机酸和细菌素等机制对具有拮抗作用，但这些相互作用的分子动力学仍未得到充分探索。本研究采用蛋白质组学方法分析共培养期间WCFS1与FRI - 1169和MN8菌株之间的相互作用。我们对在两种细菌的消耗培养基和胞质溶胶中发现的差异表达蛋白（DEP）进行了分析，揭示了不同的方向性和菌株特异性反应。研究结果表明，对的影响更为显著，有更多的DEP和上调蛋白，而显示的DEP较少且下调蛋白较多。这些菌株特异性相互作用突出了这些细菌物种之间复杂的代谢和调节调整。本研究为拮抗作用的分子机制提供了有价值的见解，并强调了蛋白质组学分析作为研究共培养系统中细菌动态的强大工具的潜力。
