Jiarpinitnun Piyarat, Iwakiri Akira, Fuke Naoyuki, Pongsawat Pornsawan, Miyanishi Chizuru, Sasaki Satomi, Taniguchi Takako, Matsui Yuto, Luangtongkum Taradon, Yamada Kentaro, Misawa Naoaki
Laboratory of Veterinary Public Health, Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Miyazaki Prefecture Tsuno Meat Hygiene Inspection Center, Miyazaki 889-1201, Japan.
Microorganisms. 2024 Nov 27;12(12):2442. doi: 10.3390/microorganisms12122442.
Spotty liver disease (SLD) affects free-range laying hens, leading to mortality and reduced egg production. species, including , have been associated with SLD cases worldwide. However, the cause of SLD-like lesions found in broilers in Japan still remains unclear. The present study aimed to investigate the involvement of spp. in broiler SLD by conducting microbiological, molecular biological, serological, histopathological, and immunohistopathological examinations using specimens of liver, bile, cecum, and serum from SLD-like and non-SLD chickens. was predominantly isolated and detected in approximately 40% of both non-SLD livers and SLD-like livers, with no significant difference between them. However, was neither isolated nor detected in this study. Gross and histopathology revealed multifocal necrotizing hepatitis, suppurative granulomatous hepatitis, and cholangiohepatitis. Hepatitis stages are classified as no hepatitis, subclinical, acute, and chronic hepatitis. was more frequently present in acute-stage SLD-like livers, and high IgG antibody levels were noted in both subclinical and SLD-like-affected chickens, indicating infection. Immunohistochemical examination also supported these findings. The findings suggest that was not involved in SLD-like in broilers in Japan, but translocation from the intestines to the liver may be a contributing factor.
斑点状肝病（SLD）影响散养产蛋母鸡，导致死亡和产蛋量下降。包括[具体物种]在内的多种物种在全球范围内都与SLD病例有关。然而，日本肉鸡中发现的类似SLD病变的病因仍不清楚。本研究旨在通过对疑似SLD和非SLD鸡的肝脏、胆汁、盲肠和血清样本进行微生物学、分子生物学、血清学、组织病理学和免疫组织病理学检查，调查[具体物种]在肉鸡SLD中的作用。[具体物种]在大约40%的非SLD肝脏和疑似SLD肝脏中均占主导地位且被检测到，两者之间无显著差异。然而，本研究中未分离和检测到[另一具体物种]。大体和组织病理学显示多灶性坏死性肝炎、化脓性肉芽肿性肝炎和胆管肝炎。肝炎阶段分为无肝炎、亚临床、急性和慢性肝炎。[具体物种]在急性阶段的疑似SLD肝脏中更常见，并且在亚临床和疑似SLD感染的鸡中均发现高IgG抗体水平，表明存在[具体物种]感染。免疫组织化学检查也支持这些发现。研究结果表明，[具体物种]与日本肉鸡的疑似SLD无关，但[具体物种]从肠道转移到肝脏可能是一个促成因素。
