Mendes Matheus B, Vidigal Pedro M P, Soto Lopez Maryoris E, Hungaro Humberto M
Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora 36036-900, MG, Brazil.
Núcleo de Análise de Biomoléculas (NuBioMol), Campus da UFV, Universidade Federal de Viçosa (UFV), Viçosa 36570-900, MG, Brazil.
Microorganisms. 2024 Dec 7;12(12):2523. doi: 10.3390/microorganisms12122523.
are significant spoilage bacteria in raw milk and dairy products, primarily due to their ability to form biofilms and resist disinfection. This study explored the effects of the phage combined with sodium hypochlorite in reducing biofilms on stainless steel at various temperatures and ages. Biofilms were formed using UFV 041 in UHT milk, incubated at 4 °C and 30 °C for 2 and 7 days. Two lytic phages were compared, with showing superior activity, reducing cell counts by 0.8 to 2.0 logs CFU/cm depending on conditions. Increasing the contact time of the phage from 4 to 8 h did not significantly affect the reduction in mature biofilms. The individual treatments of the phage and sodium hypochlorite (100 mg/L) reduced bacterial counts by 0.9 and 0.6 log CFU/cm at 30 °C, and 1.3 and 1.2 log CFU/cm at 4 °C, respectively. However, their sequential application achieved greater reductions, reaching 1.3 and 1.8 log CFU/cm for biofilms formed at 30 °C and 4 °C, respectively. These findings suggest a promising strategy for controlling in the food industry. Our findings suggest that the phage combined with chlorine improves the removal of biofilms.
是生乳和乳制品中的重要腐败细菌，主要是因为它们具有形成生物膜和抵抗消毒的能力。本研究探讨了噬菌体与次氯酸钠联合使用在不同温度和培养时间下对不锈钢表面生物膜的减少效果。使用UFV 041在超高温灭菌乳中形成生物膜，分别在4℃和30℃下培养2天和7天。比较了两种裂解性噬菌体，其中一种表现出更优异的活性，根据条件不同可使细胞数量减少0.8至2.0个对数CFU/cm²。将噬菌体的接触时间从4小时增加到8小时，对成熟生物膜的减少没有显著影响。噬菌体和次氯酸钠（100mg/L）单独处理时，在30℃下细菌数量分别减少0.9和0.6个对数CFU/cm²，在4℃下分别减少1.3和1.2个对数CFU/cm²。然而，它们的顺序应用实现了更大程度的减少，对于在30℃和4℃形成的生物膜，分别达到1.3和1.8个对数CFU/cm²。这些发现表明在食品工业中控制（此处原文缺失具体细菌名称）有一个很有前景的策略。我们的研究结果表明，噬菌体与氯联合使用可提高（此处原文缺失具体细菌名称）生物膜的去除效果。
