Dantas Leticia Ramos, Witt Maria Alice, Carneiro Everdan, Tuon Felipe Francisco
Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, Brazil.
Chemistry Department, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, Brazil.
Microorganisms. 2024 Dec 17;12(12):2616. doi: 10.3390/microorganisms12122616.
Silver nanoparticles have garnered significant attention for their antimicrobial applications. The aim of this study was to develop and characterize a silver nanoparticle-enhanced bone graft and assess its antimicrobial and antibiofilm activities. Bone granules from bovine cancellous femur were impregnated with silver nanoparticles (50 nm). The antimicrobial and antibiofilm activity was tested against various pathogens, including Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Acinetobacter baumannii, and Escherichia coli. Biocompatibility and resorption were evaluated in a mouse calvaria model. All the tested pathogens showed susceptibility to silver nanoparticles, with minimal inhibitory concentrations ranging from 0.25 to 4 mg/L. The silver nanoparticle scaffolds demonstrated a significant reduction in biofilm formation across all microorganisms. The graft exhibited a biocompatibility comparable to that of autologous bone, with reduced resorption rates. Additionally, the presence of nanoparticles did not impact radiolucency, and cytotoxicity remained minimal. Bone grafts impregnated with silver nanoparticles effectively reduce biofilm formation, suggesting their potential as a strategic material for various implant applications.
银纳米颗粒因其抗菌应用而备受关注。本研究的目的是开发并表征一种银纳米颗粒增强骨移植材料，并评估其抗菌和抗生物膜活性。将来自牛松质股骨的骨颗粒用银纳米颗粒（50纳米）浸渍。针对包括金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌、粪肠球菌、鲍曼不动杆菌和大肠杆菌在内的多种病原体测试了抗菌和抗生物膜活性。在小鼠颅骨模型中评估了生物相容性和吸收情况。所有测试病原体均对银纳米颗粒敏感，最低抑菌浓度范围为0.25至4毫克/升。银纳米颗粒支架在所有微生物中均显示出生物膜形成的显著减少。该移植材料表现出与自体骨相当的生物相容性，吸收速率降低。此外，纳米颗粒的存在不影响射线透光度，细胞毒性仍保持在最低水平。浸渍有银纳米颗粒的骨移植材料可有效减少生物膜形成，表明其作为各种植入应用的战略材料的潜力。
