Molina-Salinas Gloria María, Dzul-Beh Angel, Uc-Cachón Andrés Humberto, Dzib-Baak Haziel Eleazar, González-Sánchez Avel Adolfo, Palma-Pech Geovani Antonio, Quintal-Novelo Carlos Javier
Unidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida 97150, Yucatán, Mexico.
Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Mérida 97150, Yucatán, Mexico.
Plants (Basel). 2024 Dec 14;13(24):3498. doi: 10.3390/plants13243498.
(1) Background: Carbapenem-resistant (CBRAB) and (CBRPA) are critical and high-priority pathogens that require new therapeutic developments. Medicinal plants are valuable pharmaceutical resources. This study explored the anti-infective properties of Mayan plants, , and . (2) Methods: Plant parts were extracted using -hexane, and their ability to inhibit bacterial growth and counteract resistance mechanisms and virulence factors in CBRAB and CBRPA was assessed. GC-MS analysis of the composition of the non-polar extracts and chemometric techniques correlated the phytoconstituents with anti-infective properties. (3) Results: liana and flower extracts exhibited potent antibacterial activity against strains (MIC 15.7 to 250 µg/mL) and moderate activity against strains (MIC 250 to 1000 µg/mL). leaf extract at 1000 µg/mL reduced imipenem MIC by 2048-fold for CBRAB, and flower extract significantly inhibited catalase activity (at 62.5 µg/mL) and reduced pyocyanin production (at 1000 µg/mL). Chemometric analysis identified fatty acids, fatty acid amides, terpenes, and higher alkanes as contributors to their anti-infective properties. (4) Conclusions: This study highlights the potential of medicinal plants in the development of novel anti-infective therapies against CBRAB and CBRPA with various targets.
(1) 背景：耐碳青霉烯类鲍曼不动杆菌（CBRAB）和耐碳青霉烯类铜绿假单胞菌（CBRPA）是急需新治疗方法的关键且优先级高的病原体。药用植物是宝贵的药物资源。本研究探索了玛雅植物、[植物名称1]、[植物名称2]和[植物名称3]的抗感染特性。(2) 方法：使用正己烷提取植物部位，并评估其抑制细菌生长以及对抗CBRAB和CBRPA中耐药机制和毒力因子的能力。对非极性提取物的成分进行气相色谱 - 质谱（GC - MS）分析，并采用化学计量学技术将植物成分与抗感染特性相关联。(3) 结果：[植物名称1]藤本植物和花提取物对[细菌名称1]菌株表现出强效抗菌活性（最低抑菌浓度为15.7至250微克/毫升），对[细菌名称2]菌株表现出中等活性（最低抑菌浓度为250至1000微克/毫升）。1000微克/毫升的[植物名称2]叶提取物使CBRAB对亚胺培南的最低抑菌浓度降低了2048倍，[植物名称3]花提取物显著抑制[细菌名称3]过氧化氢酶活性（在62.5微克/毫升时）并减少[细菌名称4]绿脓菌素的产生（在1000微克/毫升时）。化学计量学分析确定脂肪酸、脂肪酸酰胺、萜类和高级烷烃是其抗感染特性的贡献成分。(4) 结论：本研究突出了药用植物在开发针对CBRAB和CBRPA具有多种靶点的新型抗感染疗法方面的潜力。
