Unidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida, Yucatán, México.
Centro de Investigación en Microbiología Ambiental y Biotecnología, Universidad Autónoma de Campeche, San Francisco de Campeche, Campeche, México.
Nat Prod Res. 2024 Oct;38(19):3444-3448. doi: 10.1080/14786419.2023.2245537. Epub 2023 Aug 11.
Mexico's Yucatan Peninsula is an endemic area of cutaneous leishmaniasis, locally known as the chiclero's ulcer, and Mayan traditional medicine which refers to the use of Radlk, known as k'an chuunup. Aqueous and organic leaves extracts were evaluated against promastigotes and amastigotes of Toxicity tests of extracts were performed using Vero and J774A.1 macrophage cell lines. The composition of the most active extracts was analysed by GC-MS. The -hexane and ethyl acetate extracts showed potent anti- activity against the promastigote form, and remarkably, -hexane extract exhibited potent activity against the amastigote form. Both extracts showed low toxicity on Vero both not on J774A.1 cells. Analysis of both bioactive extracts identified as more abundant compounds, germacrene D-4-ol and thunbergen in -hexane, and thunbergol in ethyl acetate extracts. Our study presents as anti- phytomedicine supporting its medicinal use and contributes to the understanding of its phytochemical composition.
墨西哥尤卡坦半岛是皮肤利什曼病的地方性流行区，当地俗称“chiclero 的溃疡”，玛雅传统医学中提到使用 Radlk，被称为 k'an chuunup。评估了水提物和有机溶剂提物对前鞭毛体和无鞭毛体的活性。使用 Vero 和 J774A.1 巨噬细胞系进行了提取物的毒性试验。通过 GC-MS 分析了最具活性的提取物的成分。-己烷和乙酸乙酯提取物对前鞭毛体形式表现出很强的活性，而令人惊讶的是，-己烷提取物对无鞭毛体形式也表现出很强的活性。两种提取物在 Vero 细胞上的毒性都很低，但在 J774A.1 细胞上没有。对两种生物活性提取物的分析确定了更为丰富的化合物，-己烷中的 germacrene D-4-ol 和 thunbergen，以及乙酸乙酯提取物中的 thunbergol。我们的研究为作为抗利什曼病的植物药提供了支持，支持其药用用途，并有助于理解其植物化学成分。
