Frey Carlos, Saez-Aguayo Susana, Encina Antonio, Acebes José Luis
Área de Fisiología Vegetal, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24007 León, Spain.
Instituto de Biología Molecular, Genómica y Proteómica, Universidad de León, 24007 León, Spain.
Plants (Basel). 2024 Dec 17;13(24):3519. doi: 10.3390/plants13243519.
Cell walls play essential roles in cell recognition, tissue adhesion, and wound response. In particular, pectins as cell-adhesive agents are expected to play a key role in the early stages of grafting. To test this premise, this study focused on examining the dynamics of the accumulation and degree of methyl-esterification of pectic polysaccharides at the graft junctions using tomato autografts as an experimental model. Monosaccharide analysis showed a marked increase in homogalacturonan from 25% to 32 or 34% at the junction zones early after grafting. In addition, a decrease in the degree of homogalacturonan methyl-esterification up to 38% in the scion and 64% in the rootstock was observed in the first few days after grafting, accompanied by an increase in pectin methyl-esterase activity of up to 20-30% in the tissues surrounding the graft junction. These results shed light on the role of homogalacturonan in grafting and reinforce the key function of pectin as one of the most relevant cell wall components during the grafting process.
细胞壁在细胞识别、组织黏附及伤口反应中发挥着重要作用。特别是，果胶作为细胞黏附剂有望在嫁接早期发挥关键作用。为验证这一前提，本研究以番茄自体嫁接为实验模型，着重研究嫁接部位果胶多糖的积累动态及甲酯化程度。单糖分析表明，嫁接后早期，接合区的同型半乳糖醛酸显著增加，从25%增至32%或34%。此外，嫁接后头几天，接穗中同型半乳糖醛酸甲酯化程度降低至38%，砧木中降低至64%，同时嫁接部位周围组织中的果胶甲酯酶活性增加高达20 - 30%。这些结果揭示了同型半乳糖醛酸在嫁接中的作用，并强化了果胶作为嫁接过程中最相关细胞壁成分之一的关键功能。
